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Statements

Subject Item
n2:RIV%2F67985939%3A_____%2F10%3A00357655%21RIV11-MSM-67985939
rdf:type
n15:Vysledek skos:Concept
dcterms:description
Three methods of algal assays with green alga Chlorella kessleri - the standard assay, microassay, and the proposed fluorescence assay - were compared from the point of view of reliability of EC50 detection, the minimum required time for the detection, sensitivity of individual measurement, i.e. at which cell density the particular assay can be used for EC50 estimation, and the time stability of the EC50 values. No significant differences in EC50 between the assay type and the inoculation methods were observed with the exception of the significant difference between ECc50-72 in the standard assay and ECr50 in the microassay in the standard inoculation. The ECf50 derived from variable fluorescence measurement values correspond to EC50 values derived from the growth rates. Fluorescence measurement revealed the toxic effect after 24 h of exposure (24 h before the other bioassays) at lower cell density (50%). Three methods of algal assays with green alga Chlorella kessleri - the standard assay, microassay, and the proposed fluorescence assay - were compared from the point of view of reliability of EC50 detection, the minimum required time for the detection, sensitivity of individual measurement, i.e. at which cell density the particular assay can be used for EC50 estimation, and the time stability of the EC50 values. No significant differences in EC50 between the assay type and the inoculation methods were observed with the exception of the significant difference between ECc50-72 in the standard assay and ECr50 in the microassay in the standard inoculation. The ECf50 derived from variable fluorescence measurement values correspond to EC50 values derived from the growth rates. Fluorescence measurement revealed the toxic effect after 24 h of exposure (24 h before the other bioassays) at lower cell density (50%).
dcterms:title
Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta) Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta)
skos:prefLabel
Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta) Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta)
skos:notation
RIV/67985939:_____/10:00357655!RIV11-MSM-67985939
n3:aktivita
n17:Z n17:P
n3:aktivity
P(1M0571), Z(AV0Z60050516)
n3:cisloPeriodika
6
n3:dodaniDat
n16:2011
n3:domaciTvurceVysledku
n11:5200164
n3:druhVysledku
n18:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
283906
n3:idVysledku
RIV/67985939:_____/10:00357655
n3:jazykVysledku
n5:eng
n3:klicovaSlova
bioassay; variable chlorophyll fluorescence; Chlorella kessleri
n3:klicoveSlovo
n6:variable%20chlorophyll%20fluorescence n6:bioassay n6:Chlorella%20kessleri
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[15ECC33DAB99]
n3:nazevZdroje
Environmental Toxicology
n3:obor
n8:EF
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
1
n3:projekt
n12:1M0571
n3:rokUplatneniVysledku
n16:2010
n3:svazekPeriodika
25
n3:tvurceVysledku
Kvíderová, Jana
n3:wos
000284683800003
n3:zamer
n9:AV0Z60050516
s:issn
1520-4081
s:numberOfPages
10