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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F15%3A00441399%21RIV15-GA0-67985904
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Polo-like kinase 1 (PLK1) orchestrates multiple events of cell division. Although PLK1 function has been intensively studied in centriole-containing and rapidly cycling somatic cells, much less is known about its function in the meiotic divisions of mammalian oocytes, which arrest for a long period of time in prophase before meiotic resumption and lack centrioles for spindle assembly. Here, using specific small molecule inhibition combined with live mouse oocyte imaging, we comprehensively characterize meiotic PLK1's functions. We show that PLK1 becomes activated at meiotic resumption on microtubule organizing centers (MTOCs) and later at kinetochores. PLK1 is required for efficient meiotic resumption by promoting nuclear envelope breakdown. PLK1 is also needed to recruit centrosomal proteins to acentriolar MTOCs to promote normal spindle formation, as well as for stable kinetochore-microtubule attachment. Consequently, PLK1 inhibition leads to metaphase I arrest with misaligned chromosomes activating the spindle assembly checkpoint (SAC). Unlike in mitosis, the metaphase I arrest is not bypassed by the inactivation of the SAC. We show that PLK1 is required for the full activation of the anaphase promoting complex/cyclosome (APC/C) by promoting the degradation of the APC/C inhibitor EMI1 and is therefore essential for entry into anaphase I. Moreover, our data suggest that PLK1 is required for proper chromosome segregation and the maintenance of chromosome condensation during the meiosis I-II transition, independently of the APC/C. Thus, our results define the meiotic roles of PLK1 in oocytes and reveal interesting differential requirements of PLK1 between mitosis and oocyte meiosis in mammals. Polo-like kinase 1 (PLK1) orchestrates multiple events of cell division. Although PLK1 function has been intensively studied in centriole-containing and rapidly cycling somatic cells, much less is known about its function in the meiotic divisions of mammalian oocytes, which arrest for a long period of time in prophase before meiotic resumption and lack centrioles for spindle assembly. Here, using specific small molecule inhibition combined with live mouse oocyte imaging, we comprehensively characterize meiotic PLK1's functions. We show that PLK1 becomes activated at meiotic resumption on microtubule organizing centers (MTOCs) and later at kinetochores. PLK1 is required for efficient meiotic resumption by promoting nuclear envelope breakdown. PLK1 is also needed to recruit centrosomal proteins to acentriolar MTOCs to promote normal spindle formation, as well as for stable kinetochore-microtubule attachment. Consequently, PLK1 inhibition leads to metaphase I arrest with misaligned chromosomes activating the spindle assembly checkpoint (SAC). Unlike in mitosis, the metaphase I arrest is not bypassed by the inactivation of the SAC. We show that PLK1 is required for the full activation of the anaphase promoting complex/cyclosome (APC/C) by promoting the degradation of the APC/C inhibitor EMI1 and is therefore essential for entry into anaphase I. Moreover, our data suggest that PLK1 is required for proper chromosome segregation and the maintenance of chromosome condensation during the meiosis I-II transition, independently of the APC/C. Thus, our results define the meiotic roles of PLK1 in oocytes and reveal interesting differential requirements of PLK1 between mitosis and oocyte meiosis in mammals.
dcterms:title
Multiple Requirements of PLK1 during Mouse Oocyte Maturation Multiple Requirements of PLK1 during Mouse Oocyte Maturation
skos:prefLabel
Multiple Requirements of PLK1 during Mouse Oocyte Maturation Multiple Requirements of PLK1 during Mouse Oocyte Maturation
skos:notation
RIV/67985904:_____/15:00441399!RIV15-GA0-67985904
n3:aktivita
n6:I n6:P
n3:aktivity
I, P(ED2.1.00/03.0124), P(GAP502/11/0593), P(GC301/09/J036), P(GPP301/11/P081), P(LH12057)
n3:cisloPeriodika
2
n3:dodaniDat
n15:2015
n3:domaciTvurceVysledku
n4:8523487 n4:2619458 n4:9743413 n4:5434505
n3:druhVysledku
n18:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
294
n3:idVysledku
RIV/67985904:_____/15:00441399
n3:jazykVysledku
n12:eng
n3:klicovaSlova
PLK1; meiosis; mouse oocytes
n3:klicoveSlovo
n10:mouse%20oocytes n10:meiosis n10:PLK1
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[403592D284F4]
n3:nazevZdroje
PLoS ONE
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
10
n3:projekt
n5:LH12057 n5:GPP301%2F11%2FP081 n5:GAP502%2F11%2F0593 n5:ED2.1.00%2F03.0124 n5:GC301%2F09%2FJ036
n3:rokUplatneniVysledku
n15:2015
n3:svazekPeriodika
10
n3:tvurceVysledku
Baran, V. Ellenberg, J. Kaido, M. Yoshida, S. Motlík, Jan Šolc, Petr Brzáková, Adéla Mayer, Alexandra Šámalová, P. Kitajima, T.
s:issn
1932-6203
s:numberOfPages
25
n17:doi
10.1371/journal.pone.0116783