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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F15%3A00439410%21RIV15-TA0-67985904
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Expression of doublecortin (DCX), a 43–53 kDa microtubule binding protein, is frequently used as (i) an early neuronal marker to identify the stage of neuronal maturation of in vivo grafted neuronal precursors (NSCs), and (ii) a neuronal fate marker transiently expressed by immature neurons during development. Reliable identification of the origin of DCX-immunoreactive cells (i.e., host vs. graft) requires detailed spatial and temporal mapping of endogenous DCX expression at graft-targeted brain or spinal cord regions. Accordingly, in the present study, we analyzed (i) the time course of DCX expression in pre- and postnatal rat and porcine spinal cord, and (ii) the DCX expression in spinally grafted porcine-induced pluripotent stem cells (iPS)-derived NSCs and human embryonic stem cell (ES)-derived NSCs. In addition, complementary temporospatial GFAP expression study in porcine spinal cord was also performed. In 21-day-old rat fetuses, an intense DCX immunoreactivity distributed between the dorsal horn (DH) and ventral horn was seen and was still present in the DH neurons on postnatal day 20. In animals older than 8 weeks, no DCX immunoreactivity was seen at any spinal cord laminae. In contrast to rat, in porcine spinal cord (gestational period 113–114 days), DCX was only expressed during the pre-natal period (up to 100 days) but was no longer present in newborn piglets or in adult animals. Immunohistochemical analysis was confirmed with a comparable expression profile by western blot analysis. Contrary, the expression of porcine GFAP started within 70–80 days of the pre-natal period. Spinally grafted porcine iPS-NSCs and human ES-NSCs showed clear DCX expression at 3–4 weeks postgrafting. These data indicate that in spinal grafting studies which employ postnatal or adult porcine models, the expression of DCX can be used as a reliable marker of grafted neurons. In contrast, if grafted neurons are to be analyzed during the first 4 postnatal weeks in the rat spinal... Expression of doublecortin (DCX), a 43–53 kDa microtubule binding protein, is frequently used as (i) an early neuronal marker to identify the stage of neuronal maturation of in vivo grafted neuronal precursors (NSCs), and (ii) a neuronal fate marker transiently expressed by immature neurons during development. Reliable identification of the origin of DCX-immunoreactive cells (i.e., host vs. graft) requires detailed spatial and temporal mapping of endogenous DCX expression at graft-targeted brain or spinal cord regions. Accordingly, in the present study, we analyzed (i) the time course of DCX expression in pre- and postnatal rat and porcine spinal cord, and (ii) the DCX expression in spinally grafted porcine-induced pluripotent stem cells (iPS)-derived NSCs and human embryonic stem cell (ES)-derived NSCs. In addition, complementary temporospatial GFAP expression study in porcine spinal cord was also performed. In 21-day-old rat fetuses, an intense DCX immunoreactivity distributed between the dorsal horn (DH) and ventral horn was seen and was still present in the DH neurons on postnatal day 20. In animals older than 8 weeks, no DCX immunoreactivity was seen at any spinal cord laminae. In contrast to rat, in porcine spinal cord (gestational period 113–114 days), DCX was only expressed during the pre-natal period (up to 100 days) but was no longer present in newborn piglets or in adult animals. Immunohistochemical analysis was confirmed with a comparable expression profile by western blot analysis. Contrary, the expression of porcine GFAP started within 70–80 days of the pre-natal period. Spinally grafted porcine iPS-NSCs and human ES-NSCs showed clear DCX expression at 3–4 weeks postgrafting. These data indicate that in spinal grafting studies which employ postnatal or adult porcine models, the expression of DCX can be used as a reliable marker of grafted neurons. In contrast, if grafted neurons are to be analyzed during the first 4 postnatal weeks in the rat spinal...
dcterms:title
Time Course of Spinal Doublecortin Expression in Developing Rat and Porcine Spinal Cord: Implication in In Vivo Neural Precursor Grafting Studies Time Course of Spinal Doublecortin Expression in Developing Rat and Porcine Spinal Cord: Implication in In Vivo Neural Precursor Grafting Studies
skos:prefLabel
Time Course of Spinal Doublecortin Expression in Developing Rat and Porcine Spinal Cord: Implication in In Vivo Neural Precursor Grafting Studies Time Course of Spinal Doublecortin Expression in Developing Rat and Porcine Spinal Cord: Implication in In Vivo Neural Precursor Grafting Studies
skos:notation
RIV/67985904:_____/15:00439410!RIV15-TA0-67985904
n4:aktivita
n13:I n13:P
n4:aktivity
I, P(ED2.1.00/03.0124), P(TA01011466)
n4:cisloPeriodika
1
n4:dodaniDat
n8:2015
n4:domaciTvurceVysledku
n5:5297001 n5:1947788 n5:5434505 n5:4536096 n5:6829236
n4:druhVysledku
n15:J
n4:duvernostUdaju
n18:S
n4:entitaPredkladatele
n16:predkladatel
n4:idSjednocenehoVysledku
466
n4:idVysledku
RIV/67985904:_____/15:00439410
n4:jazykVysledku
n11:eng
n4:klicovaSlova
doublecortin; spinal cord development; spinal neural precursor grafting; minipig; rat; GFAP
n4:klicoveSlovo
n6:spinal%20cord%20development n6:spinal%20neural%20precursor%20grafting n6:rat n6:GFAP n6:doublecortin n6:minipig
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[9B0F9D116BE3]
n4:nazevZdroje
Cellular and Molecular Neurobiology
n4:obor
n17:FH
n4:pocetDomacichTvurcuVysledku
5
n4:pocetTvurcuVysledku
9
n4:projekt
n12:ED2.1.00%2F03.0124 n12:TA01011466
n4:rokUplatneniVysledku
n8:2015
n4:svazekPeriodika
35
n4:tvurceVysledku
Juhás, Štefan Holubová, Monika Strnádel, Ján Hruška-Plocháň, M. Marsala, M. Motlík, Jan Marsala, S. Juhásová, Jana Doležalová, D.
n4:wos
000347884500007
s:issn
0272-4340
s:numberOfPages
14
n9:doi
10.1007/s10571-014-0145-7