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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F14%3A00427357%21RIV15-GA0-67985904
rdf:type
skos:Concept n17:Vysledek
dcterms:description
This study was designed to determine whether inhibition of either cyclooxygenase-2 (COX-2) by indomethacin or progesterone receptor (PR) by PR antagonist, RU486, affects oocyte maturation, progesterone production, and covalent binding between hyaluronan (HA) and heavy chains of inter-α trypsin inhibitor, as well as expression of cumulus expansion-associated proteins (HA-binding protein, tumor necrosis factor α-induced protein 6, pentraxin 3) in oocyte-cumulus complexes (OCCs). The experiments were based on freshly isolated porcine OCC cultures in which the consequences of PR and COX-2 inhibition on the final processes of oocyte maturation were determined. Granulosa cells (GCs) and OCCs were cultured in medium supplemented with FSH/LH (both 100ng/mL) in the presence/absence of RU486 or indomethacin. Western blot analysis, 3H-glucosamine hydrochloride assay, immunofluorescence, and radioimmunoassay were performed. Only treatment with RU486 (25 μM) caused a decrease in the number of oocytes that reached germinal vesicle breakdown and metaphase II stage compared with indomethacin (100μM) or FSH/LH treatment alone after 44h. All treated OCCs synthesized an almost equal amount of HA. Heavy chains (of inter-α trypsin inhibitor)-HA covalent complexes were formed during invitro FSH/LH-stimulated expansion in RU486- or indomethacin-treated OCCs. Follicle-stimulating hormone/LH-induced progesterone production by OCCs was increased in the presence of RU486 after 44h. In contrast, a decrease of FSH/LH-stimulated progesterone production by GCs was detected in the presence of either RU486 or indomethacin after 72h. We suggest that the PR-dependent pathway may be involved in the regulation of oocyte maturation. Both PR and COX-2 regulate FSH/LH-stimulated progesterone production by OCCs and GCs. This study was designed to determine whether inhibition of either cyclooxygenase-2 (COX-2) by indomethacin or progesterone receptor (PR) by PR antagonist, RU486, affects oocyte maturation, progesterone production, and covalent binding between hyaluronan (HA) and heavy chains of inter-α trypsin inhibitor, as well as expression of cumulus expansion-associated proteins (HA-binding protein, tumor necrosis factor α-induced protein 6, pentraxin 3) in oocyte-cumulus complexes (OCCs). The experiments were based on freshly isolated porcine OCC cultures in which the consequences of PR and COX-2 inhibition on the final processes of oocyte maturation were determined. Granulosa cells (GCs) and OCCs were cultured in medium supplemented with FSH/LH (both 100ng/mL) in the presence/absence of RU486 or indomethacin. Western blot analysis, 3H-glucosamine hydrochloride assay, immunofluorescence, and radioimmunoassay were performed. Only treatment with RU486 (25 μM) caused a decrease in the number of oocytes that reached germinal vesicle breakdown and metaphase II stage compared with indomethacin (100μM) or FSH/LH treatment alone after 44h. All treated OCCs synthesized an almost equal amount of HA. Heavy chains (of inter-α trypsin inhibitor)-HA covalent complexes were formed during invitro FSH/LH-stimulated expansion in RU486- or indomethacin-treated OCCs. Follicle-stimulating hormone/LH-induced progesterone production by OCCs was increased in the presence of RU486 after 44h. In contrast, a decrease of FSH/LH-stimulated progesterone production by GCs was detected in the presence of either RU486 or indomethacin after 72h. We suggest that the PR-dependent pathway may be involved in the regulation of oocyte maturation. Both PR and COX-2 regulate FSH/LH-stimulated progesterone production by OCCs and GCs.
dcterms:title
Effects of RU486 and indomethacin on meiotic maturation, formation of extracellular matrix, and progesterone production by porcine oocyte-cumulus complexes Effects of RU486 and indomethacin on meiotic maturation, formation of extracellular matrix, and progesterone production by porcine oocyte-cumulus complexes
skos:prefLabel
Effects of RU486 and indomethacin on meiotic maturation, formation of extracellular matrix, and progesterone production by porcine oocyte-cumulus complexes Effects of RU486 and indomethacin on meiotic maturation, formation of extracellular matrix, and progesterone production by porcine oocyte-cumulus complexes
skos:notation
RIV/67985904:_____/14:00427357!RIV15-GA0-67985904
n4:aktivita
n9:I n9:P
n4:aktivity
I, P(GAP502/11/0593)
n4:cisloPeriodika
1
n4:dodaniDat
n13:2015
n4:domaciTvurceVysledku
n15:6729029 n15:3887103
n4:druhVysledku
n8:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n6:predkladatel
n4:idSjednocenehoVysledku
13766
n4:idVysledku
RIV/67985904:_____/14:00427357
n4:jazykVysledku
n18:eng
n4:klicovaSlova
RU486; indomethacin; oocyte; granulosa; progesterone
n4:klicoveSlovo
n10:progesterone n10:indomethacin n10:granulosa n10:oocyte n10:RU486
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[51F98D9A5B75]
n4:nazevZdroje
Domestic Animal Endocrinology
n4:obor
n16:ED
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
4
n4:projekt
n11:GAP502%2F11%2F0593
n4:rokUplatneniVysledku
n13:2014
n4:svazekPeriodika
48
n4:tvurceVysledku
Nagyová, Eva Scsuková, S. Mlynarčíková, A. Kalous, Jaroslav
n4:wos
000337643900002
s:issn
0739-7240
s:numberOfPages
8
n5:doi
10.1016/j.domaniend.2014.01.003