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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F13%3A00393917%21RIV14-TA0-67985904
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Achievement of effective, safe and long-term immunosuppression represents one of the challenges in experimental allogeneic and xenogeneic cell and organ transplantation. The goal of the present study was to develop a reliable, long-term immunosuppression protocol in Sprague-Dawley (SD) rats by: 1) comparing the pharmacokinetics of four different subcutaneously delivered/implanted tacrolimus (TAC) formulations, including: i) caster oil/saline solution, ii) unilamellar or multilamellar liposomes, iii) biodegradable microspheres, and iv) biodegradable 3-month lasting pellets; and 2) defining the survival and immune response in animals receiving spinal injections of human neural precursors at 6. weeks to 3. months after cell grafting. In animals implanted with TAC pellets (3.4. mg/kg/day), a stable 3-month lasting plasma concentration of TAC averaging 19.1. . 4.9. ng/ml was measured. Analysis of grafted cell survival in SOD. + or spinal trauma-injured SD rats immunosuppressed with 3-month lasting TAC pellets (3.4-5.1. mg/kg/day) showed the consistent presence of implanted human neurons with minimal or no local T-cell infiltration. These data demonstrate that the use of TAC pellets can represent an effective, long-lasting immunosuppressive drug delivery system that is safe, simple to implement and is associated with a long-term human neural precursor survival after grafting into the spinal cord of SOD. + or spinal trauma-injured SD rats. Achievement of effective, safe and long-term immunosuppression represents one of the challenges in experimental allogeneic and xenogeneic cell and organ transplantation. The goal of the present study was to develop a reliable, long-term immunosuppression protocol in Sprague-Dawley (SD) rats by: 1) comparing the pharmacokinetics of four different subcutaneously delivered/implanted tacrolimus (TAC) formulations, including: i) caster oil/saline solution, ii) unilamellar or multilamellar liposomes, iii) biodegradable microspheres, and iv) biodegradable 3-month lasting pellets; and 2) defining the survival and immune response in animals receiving spinal injections of human neural precursors at 6. weeks to 3. months after cell grafting. In animals implanted with TAC pellets (3.4. mg/kg/day), a stable 3-month lasting plasma concentration of TAC averaging 19.1. . 4.9. ng/ml was measured. Analysis of grafted cell survival in SOD. + or spinal trauma-injured SD rats immunosuppressed with 3-month lasting TAC pellets (3.4-5.1. mg/kg/day) showed the consistent presence of implanted human neurons with minimal or no local T-cell infiltration. These data demonstrate that the use of TAC pellets can represent an effective, long-lasting immunosuppressive drug delivery system that is safe, simple to implement and is associated with a long-term human neural precursor survival after grafting into the spinal cord of SOD. + or spinal trauma-injured SD rats.
dcterms:title
Effective long-term immunosuppression in rats by subcutaneously implanted sustained-release tacrolimus pellet: Effect on spinally grafted human neural precursor survival Effective long-term immunosuppression in rats by subcutaneously implanted sustained-release tacrolimus pellet: Effect on spinally grafted human neural precursor survival
skos:prefLabel
Effective long-term immunosuppression in rats by subcutaneously implanted sustained-release tacrolimus pellet: Effect on spinally grafted human neural precursor survival Effective long-term immunosuppression in rats by subcutaneously implanted sustained-release tacrolimus pellet: Effect on spinally grafted human neural precursor survival
skos:notation
RIV/67985904:_____/13:00393917!RIV14-TA0-67985904
n5:predkladatel
n10:ico%3A67985904
n3:aktivita
n14:I n14:P
n3:aktivity
I, P(ED2.1.00/03.0124), P(TA01011466)
n3:cisloPeriodika
October
n3:dodaniDat
n13:2014
n3:domaciTvurceVysledku
n6:1947788 n6:5434505 n6:4536096 n6:8103283
n3:druhVysledku
n17:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
71865
n3:idVysledku
RIV/67985904:_____/13:00393917
n3:jazykVysledku
n12:eng
n3:klicovaSlova
immunosuppression; xenograft; human neural precursors
n3:klicoveSlovo
n15:human%20neural%20precursors n15:immunosuppression n15:xenograft
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[4E41C7970B5C]
n3:nazevZdroje
Experimental Neurology
n3:obor
n18:FH
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
19
n3:projekt
n8:ED2.1.00%2F03.0124 n8:TA01011466
n3:rokUplatneniVysledku
n13:2013
n3:svazekPeriodika
248
n3:tvurceVysledku
Motlík, Jan Johe, K. Rypáček, František Yamada, K. Bui, J. D. Marsala, S. Machová, Luďka Leerink, M. Hefferan, M. P. Ševc, J. Juhás, Štefan Juhásová, Jana Lukáčová, N. Goldberg, D. Marsala, M. Hruška-Plocháň, Marian van Gorp, S. Kakinohana, O. Santucci, C.
n3:wos
000327684600009
s:issn
0014-4886
s:numberOfPages
15
n11:doi
10.1016/j.expneurol.2013.05.017