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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F13%3A00389657%21RIV13-GA0-67985904
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Background: Sympatric species pairs are particularly common in freshwater fishes associated with postglacial lakes in northern temperate environments. The nature of divergences between co-occurring sympatric species, factors contributing to reproductive isolation and modes of genome evolution is a much debated topic in evolutionary biology addressed by various experimental tools. To the best of our knowledge, nobody approached this field using molecular cytogenetics. We examined chromosomes and genomes of one postglacial species pair, sympatric European winter-spawning Coregonus albula and the local endemic dwarf-sized spring-spawning C. fontanae, both originating in Lake Stechlin. We have employed molecular cytogenetic tools to identify the genomic differences between the two species of the sympatric pair on the sub-chromosomal level of resolution. Results: Fluorescence in situ hybridization (FISH) experiments consistently revealed a distinct variation in the copy number of loci of the major ribosomal DNA (the 45S unit) between C. albula and C. fontanae genomes. In C. fontanae, up to 40 chromosomes were identified to bear a part of the major ribosomal DNA, while in C. albula only 8--10 chromosomes possessed these genes. To determine mechanisms how such extensive genome alternation might have arisen, a PCR screening for retrotransposons from genomic DNA of both species was performed. The amplified retrotransposon Rex1 was used as a probe for FISH mapping onto chromosomes of both species. These experiments showed a clear co-localization of the ribosomal DNA and the retrotransposon Rex1 in a pericentromeric region of one or two acrocentric chromosomes in both species. Conclusion: We demonstrated genomic consequences of a rapid ecological speciation on the level undetectable by neither sequence nor karyotype analysis. We provide indirect evidence that ribosomal DNA probably utilized the spreading mechanism of retrotransposons subsequently affecting recombination rat... Background: Sympatric species pairs are particularly common in freshwater fishes associated with postglacial lakes in northern temperate environments. The nature of divergences between co-occurring sympatric species, factors contributing to reproductive isolation and modes of genome evolution is a much debated topic in evolutionary biology addressed by various experimental tools. To the best of our knowledge, nobody approached this field using molecular cytogenetics. We examined chromosomes and genomes of one postglacial species pair, sympatric European winter-spawning Coregonus albula and the local endemic dwarf-sized spring-spawning C. fontanae, both originating in Lake Stechlin. We have employed molecular cytogenetic tools to identify the genomic differences between the two species of the sympatric pair on the sub-chromosomal level of resolution. Results: Fluorescence in situ hybridization (FISH) experiments consistently revealed a distinct variation in the copy number of loci of the major ribosomal DNA (the 45S unit) between C. albula and C. fontanae genomes. In C. fontanae, up to 40 chromosomes were identified to bear a part of the major ribosomal DNA, while in C. albula only 8--10 chromosomes possessed these genes. To determine mechanisms how such extensive genome alternation might have arisen, a PCR screening for retrotransposons from genomic DNA of both species was performed. The amplified retrotransposon Rex1 was used as a probe for FISH mapping onto chromosomes of both species. These experiments showed a clear co-localization of the ribosomal DNA and the retrotransposon Rex1 in a pericentromeric region of one or two acrocentric chromosomes in both species. Conclusion: We demonstrated genomic consequences of a rapid ecological speciation on the level undetectable by neither sequence nor karyotype analysis. We provide indirect evidence that ribosomal DNA probably utilized the spreading mechanism of retrotransposons subsequently affecting recombination rat...
dcterms:title
Genome differentiation in a species pair of coregonine fishes: An extremely rapid speciation driven by stress-activated retrotransposons mediating extensive ribosomal DNA multiplications Genome differentiation in a species pair of coregonine fishes: An extremely rapid speciation driven by stress-activated retrotransposons mediating extensive ribosomal DNA multiplications
skos:prefLabel
Genome differentiation in a species pair of coregonine fishes: An extremely rapid speciation driven by stress-activated retrotransposons mediating extensive ribosomal DNA multiplications Genome differentiation in a species pair of coregonine fishes: An extremely rapid speciation driven by stress-activated retrotransposons mediating extensive ribosomal DNA multiplications
skos:notation
RIV/67985904:_____/13:00389657!RIV13-GA0-67985904
n3:predkladatel
n11:ico%3A67985904
n6:aktivita
n16:P n16:Z
n6:aktivity
P(GA523/08/0824), P(GPP506/11/P596), P(LC06073), Z(AV0Z50450515)
n6:cisloPeriodika
1
n6:dodaniDat
n8:2013
n6:domaciTvurceVysledku
n7:5010411 n7:7356625 n7:3635155 n7:1105795 n7:9818863 Bohlen, Jörg
n6:druhVysledku
n13:J
n6:duvernostUdaju
n19:S
n6:entitaPredkladatele
n18:predkladatel
n6:idSjednocenehoVysledku
76419
n6:idVysledku
RIV/67985904:_____/13:00389657
n6:jazykVysledku
n12:eng
n6:klicovaSlova
species pairs
n6:klicoveSlovo
n14:species%20pairs
n6:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n6:kontrolniKodProRIV
[93D6583AAF39]
n6:nazevZdroje
BMC Evolutionary Biology
n6:obor
n15:EB
n6:pocetDomacichTvurcuVysledku
6
n6:pocetTvurcuVysledku
8
n6:projekt
n10:GA523%2F08%2F0824 n10:GPP506%2F11%2FP596 n10:LC06073
n6:rokUplatneniVysledku
n8:2013
n6:svazekPeriodika
13
n6:tvurceVysledku
Bohlen, Jörg Sember, Alexandr Freyhof, J. Symonová, Radka Staaks, G.B.O. Rábová, Marie Ráb, Petr Majtánová, Zuzana
n6:zamer
n20:AV0Z50450515
s:issn
1471-2148
s:numberOfPages
21
n17:doi
10.1186/1471-2148-13-42