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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F12%3A00387319%21RIV13-AV0-67985904
rdf:type
n15:Vysledek skos:Concept
dcterms:description
In previous studies, we have demonstrated that spinal grafting of human or rat fetal spinal neural precursors leads to amelioration of spasticity and improvement in ambulatory function in rats with spinal ischemic injury. In the current study, we characterize the survival and maturation of three different human embryonic stem (ES) cell line-derived neural precursors (hNPCs) once grafted into ischemia-injured lumbar spinal cord in rats or in naive immunosuppressed minipigs. Proliferating HUES-2, HUES-7 or HUES-9 colonies were induced to form embryoid bodies. During the nestinpositive stage, the rosettes were removed and CD184+/CD271-/CD44-/CD24+ population of ES-hNPCs FAC-sorted and expanded. Male Sprague-Dawley rats with spinal ischemic injury or naïve immunosuppressed Gottingen-Minnesota minipigs received 10 bilateral injections of ES-NPCs into the L2-L5 gray matter. After cell grafting, animals survived for 2 weeks to 4.5 months and the presence of grafted cells was confirmed after staining spinal cord sections with a combination of human-specific (hNUMA, HO14, hNSE, hSYN) or non-specific (DCX, MAP2, CHAT, GFAP, APC) antibodies. In the majority of grafted animals, hNUMA-positive grafted cells were identified. At 2-4 weeks after grafting, double-labeled hNUMA/DCX immunoreactive neurons were seen with extensive DCX+ processes. At survival intervals of 4-8 weeks, hNSE+ neurons and expression of hSYN was identified. Some hSYN positive terminals formed putative synapses with the host neurons.Quantitative analysis of hNUMA+ cells at 2 months after grafting showed comparable cell survival for all 3 cell lines. In the presence of low-level immunosuppression, no grafted cell survival was seen at 4.5 months after grafting. Spinal grafting of proliferating pluripotent HUES-7 cells led to consistent teratoma formation at 2-6 weeks after cell transplantation. These data show that ES-derived, FAC-sorted NPCs can represent an effective source of human NPCs to be used in CNS c... In previous studies, we have demonstrated that spinal grafting of human or rat fetal spinal neural precursors leads to amelioration of spasticity and improvement in ambulatory function in rats with spinal ischemic injury. In the current study, we characterize the survival and maturation of three different human embryonic stem (ES) cell line-derived neural precursors (hNPCs) once grafted into ischemia-injured lumbar spinal cord in rats or in naive immunosuppressed minipigs. Proliferating HUES-2, HUES-7 or HUES-9 colonies were induced to form embryoid bodies. During the nestinpositive stage, the rosettes were removed and CD184+/CD271-/CD44-/CD24+ population of ES-hNPCs FAC-sorted and expanded. Male Sprague-Dawley rats with spinal ischemic injury or naïve immunosuppressed Gottingen-Minnesota minipigs received 10 bilateral injections of ES-NPCs into the L2-L5 gray matter. After cell grafting, animals survived for 2 weeks to 4.5 months and the presence of grafted cells was confirmed after staining spinal cord sections with a combination of human-specific (hNUMA, HO14, hNSE, hSYN) or non-specific (DCX, MAP2, CHAT, GFAP, APC) antibodies. In the majority of grafted animals, hNUMA-positive grafted cells were identified. At 2-4 weeks after grafting, double-labeled hNUMA/DCX immunoreactive neurons were seen with extensive DCX+ processes. At survival intervals of 4-8 weeks, hNSE+ neurons and expression of hSYN was identified. Some hSYN positive terminals formed putative synapses with the host neurons.Quantitative analysis of hNUMA+ cells at 2 months after grafting showed comparable cell survival for all 3 cell lines. In the presence of low-level immunosuppression, no grafted cell survival was seen at 4.5 months after grafting. Spinal grafting of proliferating pluripotent HUES-7 cells led to consistent teratoma formation at 2-6 weeks after cell transplantation. These data show that ES-derived, FAC-sorted NPCs can represent an effective source of human NPCs to be used in CNS c...
dcterms:title
Survival and differentiation of human embryonic stem cell-derived neural precursors grafted spinally in spinal ischemia-injured rats or in naive immunosuppressed minipigs: a qualitative and quantitative study Survival and differentiation of human embryonic stem cell-derived neural precursors grafted spinally in spinal ischemia-injured rats or in naive immunosuppressed minipigs: a qualitative and quantitative study
skos:prefLabel
Survival and differentiation of human embryonic stem cell-derived neural precursors grafted spinally in spinal ischemia-injured rats or in naive immunosuppressed minipigs: a qualitative and quantitative study Survival and differentiation of human embryonic stem cell-derived neural precursors grafted spinally in spinal ischemia-injured rats or in naive immunosuppressed minipigs: a qualitative and quantitative study
skos:notation
RIV/67985904:_____/12:00387319!RIV13-AV0-67985904
n15:predkladatel
n16:ico%3A67985904
n3:aktivita
n11:Z n11:P
n3:aktivity
P(1M0538), P(TA01011466), Z(AV0Z50450515)
n3:cisloPeriodika
12
n3:dodaniDat
n10:2013
n3:domaciTvurceVysledku
n8:4536096 n8:1947788 n8:5434505
n3:druhVysledku
n14:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
172618
n3:idVysledku
RIV/67985904:_____/12:00387319
n3:jazykVysledku
n19:eng
n3:klicovaSlova
spinal cord ischemia; human embryonic stem (ES) cells; neuronal precursors (NPCs)
n3:klicoveSlovo
n6:neuronal%20precursors%20%28NPCs%29 n6:spinal%20cord%20ischemia n6:human%20embryonic%20stem%20%28ES%29%20cells
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[2E860AE995C6]
n3:nazevZdroje
Cell Transplantation
n3:obor
n17:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
19
n3:projekt
n18:1M0538 n18:TA01011466
n3:rokUplatneniVysledku
n10:2012
n3:svazekPeriodika
21
n3:tvurceVysledku
Lazar, P. Galik, J. Ciacci, J. D. Keshavarzi, S. Juhásová, Jana Motlík, Jan Maršala, S. Goldberg, D. Maršala, M. Leerink, M. Vidal, J. G. Miyanohara, A. Kakinohana, O. Yuan, S. H. Juhás, Štefan Platoshyn, O. Van Gorp, S. Hefferan, M. P. Carson, C. T.
n3:zamer
n20:AV0Z50450515
s:issn
0963-6897
s:numberOfPages
17
n5:doi
10.3727/096368912X653200