This HTML5 document contains 65 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n15http://linked.opendata.cz/resource/domain/vavai/projekt/
n11http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n20http://linked.opendata.cz/resource/domain/vavai/subjekt/
n10http://linked.opendata.cz/ontology/domain/vavai/
n13http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n17http://bibframe.org/vocab/
n19http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F67985904%3A_____%2F12%3A00387297%21RIV13-AV0-67985904/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n7http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n16http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n8http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F12%3A00387297%21RIV13-AV0-67985904
rdf:type
skos:Concept n10:Vysledek
dcterms:description
Considering an analogy between wound healing and tumour progression, we studied chemokine and cytokine transcription and expression in normal fibroblasts by co-culture and in situ. Whole-genome transcriptome profiling revealed strong upregulation for the interleukin (IL)-6, IL-8 and the chemokine CXCL-1 in in vitro co-cultures of normal fibroblasts with either normal or malignant epithelial cells compared to fibroblast cultures. The same ILs/chemokines were distinctly upregulated in clinical samples of squamous cell carcinoma when compared with paired normal mucosae. Analysis of culture supernatants showed that during the course of co-culture of the fibroblasts with the epithelial cells, IL-6, IL-8 and CXCL-1 were secreted to the culture medium. Experiments with addition of any of the proteins to the culture medium supported the notion that these ILs/chemokines strongly contributed to maintenance of a low-differentiation phenotype of epithelial cells, evaluated by the detection of keratin-8. Simultaneous addition of all factors increased the extent of the effect. These studies were extended by experiments with epithelial cells, either cultured in medium conditioned by preceding use for malignant keratinocytes without and in the presence of normal or cancer-associated fibroblasts or medium containing antibodies against IL-6, IL-8 and CXCL-1. Our results indicate an analogy between wound healing and tumour growth, support the importance of epithelialmesenchymal interaction in this model system and establish a potential bio-inspired anticancer therapy. Considering an analogy between wound healing and tumour progression, we studied chemokine and cytokine transcription and expression in normal fibroblasts by co-culture and in situ. Whole-genome transcriptome profiling revealed strong upregulation for the interleukin (IL)-6, IL-8 and the chemokine CXCL-1 in in vitro co-cultures of normal fibroblasts with either normal or malignant epithelial cells compared to fibroblast cultures. The same ILs/chemokines were distinctly upregulated in clinical samples of squamous cell carcinoma when compared with paired normal mucosae. Analysis of culture supernatants showed that during the course of co-culture of the fibroblasts with the epithelial cells, IL-6, IL-8 and CXCL-1 were secreted to the culture medium. Experiments with addition of any of the proteins to the culture medium supported the notion that these ILs/chemokines strongly contributed to maintenance of a low-differentiation phenotype of epithelial cells, evaluated by the detection of keratin-8. Simultaneous addition of all factors increased the extent of the effect. These studies were extended by experiments with epithelial cells, either cultured in medium conditioned by preceding use for malignant keratinocytes without and in the presence of normal or cancer-associated fibroblasts or medium containing antibodies against IL-6, IL-8 and CXCL-1. Our results indicate an analogy between wound healing and tumour growth, support the importance of epithelialmesenchymal interaction in this model system and establish a potential bio-inspired anticancer therapy.
dcterms:title
Upregulation of IL-6, IL-8 and CXCL-1 production in dermal fibroblasts by normal/malignant epithelial cells in vitro: Immunohistochemical and transcriptomic analyses Upregulation of IL-6, IL-8 and CXCL-1 production in dermal fibroblasts by normal/malignant epithelial cells in vitro: Immunohistochemical and transcriptomic analyses
skos:prefLabel
Upregulation of IL-6, IL-8 and CXCL-1 production in dermal fibroblasts by normal/malignant epithelial cells in vitro: Immunohistochemical and transcriptomic analyses Upregulation of IL-6, IL-8 and CXCL-1 production in dermal fibroblasts by normal/malignant epithelial cells in vitro: Immunohistochemical and transcriptomic analyses
skos:notation
RIV/67985904:_____/12:00387297!RIV13-AV0-67985904
n10:predkladatel
n20:ico%3A67985904
n3:aktivita
n16:Z n16:S n16:P n16:I
n3:aktivity
I, P(NT13488), S, Z(AV0Z50450515), Z(AV0Z50520514)
n3:cisloPeriodika
12
n3:dodaniDat
n8:2013
n3:domaciTvurceVysledku
n11:5434505 n11:1147633 n11:4640225
n3:druhVysledku
n18:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
176172
n3:idVysledku
RIV/67985904:_____/12:00387297
n3:jazykVysledku
n4:eng
n3:klicovaSlova
CXCL-1; IL-6; IL-8; squamous cell carcinoma; wound healing
n3:klicoveSlovo
n7:IL-6 n7:IL-8 n7:squamous%20cell%20carcinoma n7:wound%20healing n7:CXCL-1
n3:kodStatuVydavatele
FR - Francouzská republika
n3:kontrolniKodProRIV
[680603377C60]
n3:nazevZdroje
Biology of the Cell
n3:obor
n12:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
18
n3:projekt
n15:NT13488
n3:rokUplatneniVysledku
n8:2012
n3:svazekPeriodika
104
n3:tvurceVysledku
Gabius, H. J. Pačes, Jan Strnad, Hynek Lacina, L. Fík, Z. Chovanec, M. Szabo, Pavol Dvořánková, Barbora Vlček, Čestmír Kolář, Michal Jarkovská, Karla Šáchová, Jana Motlík, Jan Betka, J. Plzák, J. Smetana, K. Kovářová, Hana Čada, Z.
n3:wos
000311910700006
n3:zamer
n13:AV0Z50520514 n13:AV0Z50450515
s:issn
0248-4900
s:numberOfPages
14
n17:doi
10.1111/boc.201200018