This HTML5 document contains 56 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n13http://linked.opendata.cz/resource/domain/vavai/projekt/
n6http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n11http://linked.opendata.cz/resource/domain/vavai/subjekt/
n10http://linked.opendata.cz/ontology/domain/vavai/
n8http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
n19http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n15http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n20http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F67985904%3A_____%2F12%3A00384274%21RIV13-GA0-67985904/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n7http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n14http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F12%3A00384274%21RIV13-GA0-67985904
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Aurora kinase A (AURKA) is an important mitotic kinase involved in the G2/M transition, centrosome maturation and separation, and spindle formation in somatic cells. We used transgenic models that specifically overexpress in mouse oocytes either wild-type (WT-AURKA) or a catalytically inactive (kinasedead) (KD-AURKA) AURKA to gain new insights regarding the role of AURKA during oocyte maturation. AURKA activation occurs shortly after hCG administration that initiates maturation in vivo. Although AURKA activity is increased in WT-AURKA oocytes, resumption of meiosis is not observed in the absence of hCG administration. Control oocytes contain one to three microtubule organizing centers (MTOCs; centrosome equivalent) at prophase I. At the time of germinal vesicle breakdown (GVBD), the first visible marker of resumption of meiosis, the MTOC number increases. In WT-AURKA oocytes, the increase in MTOC number occurs prematurely but transiently without GVBD, whereas the increase in MTOC number does not occur in control and KD-AURKA oocytes. AURKA activation is biphasic with the initial activation not requiring CDC25BCDK1 activity, whereas full activation, which is essential for the increase in MTOCs number, depends on CDK1 activity. AURKA activity also influences spindle length and regulates, independent of its protein kinase activity, the amount of MTOC associated with gamma-tubulin. Both WT-AURKA and KDAURKA transgenic mice have normal fertility during first 6 mo of life. These results suggest that although AURKA is not a trigger kinase for G2/M transition in mouse oocytes, it regulates MTOC number and spindle length, and,independent of its protein kinase activity, gamma-tubulin recruitment to MTOCs. Aurora kinase A (AURKA) is an important mitotic kinase involved in the G2/M transition, centrosome maturation and separation, and spindle formation in somatic cells. We used transgenic models that specifically overexpress in mouse oocytes either wild-type (WT-AURKA) or a catalytically inactive (kinasedead) (KD-AURKA) AURKA to gain new insights regarding the role of AURKA during oocyte maturation. AURKA activation occurs shortly after hCG administration that initiates maturation in vivo. Although AURKA activity is increased in WT-AURKA oocytes, resumption of meiosis is not observed in the absence of hCG administration. Control oocytes contain one to three microtubule organizing centers (MTOCs; centrosome equivalent) at prophase I. At the time of germinal vesicle breakdown (GVBD), the first visible marker of resumption of meiosis, the MTOC number increases. In WT-AURKA oocytes, the increase in MTOC number occurs prematurely but transiently without GVBD, whereas the increase in MTOC number does not occur in control and KD-AURKA oocytes. AURKA activation is biphasic with the initial activation not requiring CDC25BCDK1 activity, whereas full activation, which is essential for the increase in MTOCs number, depends on CDK1 activity. AURKA activity also influences spindle length and regulates, independent of its protein kinase activity, the amount of MTOC associated with gamma-tubulin. Both WT-AURKA and KDAURKA transgenic mice have normal fertility during first 6 mo of life. These results suggest that although AURKA is not a trigger kinase for G2/M transition in mouse oocytes, it regulates MTOC number and spindle length, and,independent of its protein kinase activity, gamma-tubulin recruitment to MTOCs.
dcterms:title
Aurora kinase a drives mtoc biogenesis but does not trigger resumption of meiosis in mouse oocytes matured in vivo Aurora kinase a drives mtoc biogenesis but does not trigger resumption of meiosis in mouse oocytes matured in vivo
skos:prefLabel
Aurora kinase a drives mtoc biogenesis but does not trigger resumption of meiosis in mouse oocytes matured in vivo Aurora kinase a drives mtoc biogenesis but does not trigger resumption of meiosis in mouse oocytes matured in vivo
skos:notation
RIV/67985904:_____/12:00384274!RIV13-GA0-67985904
n10:predkladatel
n11:ico%3A67985904
n4:aktivita
n12:P n12:Z
n4:aktivity
P(GPP301/11/P081), P(LH12057), P(ME08030), Z(AV0Z50450515)
n4:cisloPeriodika
4
n4:dodaniDat
n14:2013
n4:domaciTvurceVysledku
n6:2119145 n6:9643818 n6:8523487 n6:2619458 n6:5434505 n6:9991468 n6:7404867
n4:druhVysledku
n7:J
n4:duvernostUdaju
n5:S
n4:entitaPredkladatele
n20:predkladatel
n4:idSjednocenehoVysledku
124108
n4:idVysledku
RIV/67985904:_____/12:00384274
n4:jazykVysledku
n17:eng
n4:klicovaSlova
γ-tubulin; AURKA; CDC25B
n4:klicoveSlovo
n15:CDC25B n15:%CE%B3-tubulin n15:AURKA
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[8E2F237E6D0A]
n4:nazevZdroje
Biology of Reproduction
n4:obor
n9:EB
n4:pocetDomacichTvurcuVysledku
7
n4:pocetTvurcuVysledku
8
n4:projekt
n13:LH12057 n13:GPP301%2F11%2FP081 n13:ME08030
n4:rokUplatneniVysledku
n14:2012
n4:svazekPeriodika
87
n4:tvurceVysledku
Šolc, Petr Šašková, Adéla Mayer, Alexandra Böhmová, Tereza Schultz, R. M. Baran, Vladimír Motlík, Jan Panenková, Gabriela
n4:wos
000314231000009
n4:zamer
n8:AV0Z50450515
s:issn
0006-3363
s:numberOfPages
12
n19:doi
10.1095/biolreprod.112.101014