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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F11%3A00367748%21RIV12-AV0-67985904
rdf:type
skos:Concept n13:Vysledek
dcterms:description
Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteogenic differentiation of miniature pig MSCs and markers of this differentiation in vitro. Flow-cytometrically characterized MSCs were seeded on cultivation plastic (collagen I and vitronectin coated/uncoated) or plasma clot (PC)/plasma-alginate clot (PAC) scaffolds and differentiated in osteogenic medium. During three weeks of differentiation, the formation of nodules and deposition of calcium were visualized by Alizarin Red Staining. In addition, the production of alkaline phosphatase (ALP) activity was quantitatively detected by fluorescence. The expression of osteopontin, osteonectin and osteocalcin were assayed by immunohistochemistry and Western Blot analysis. We revealed a decrease of osteopontin expression in 2D and 3D environment during differentiation. Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteogenic differentiation of miniature pig MSCs and markers of this differentiation in vitro. Flow-cytometrically characterized MSCs were seeded on cultivation plastic (collagen I and vitronectin coated/uncoated) or plasma clot (PC)/plasma-alginate clot (PAC) scaffolds and differentiated in osteogenic medium. During three weeks of differentiation, the formation of nodules and deposition of calcium were visualized by Alizarin Red Staining. In addition, the production of alkaline phosphatase (ALP) activity was quantitatively detected by fluorescence. The expression of osteopontin, osteonectin and osteocalcin were assayed by immunohistochemistry and Western Blot analysis. We revealed a decrease of osteopontin expression in 2D and 3D environment during differentiation.
dcterms:title
Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment
skos:prefLabel
Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment
skos:notation
RIV/67985904:_____/11:00367748!RIV12-AV0-67985904
n13:predkladatel
n14:ico%3A67985904
n3:aktivita
n18:P n18:Z
n3:aktivity
P(1M0538), P(2B06130), Z(AV0Z50450515)
n3:cisloPeriodika
3
n3:dodaniDat
n7:2012
n3:domaciTvurceVysledku
n4:6829236 n4:5297001 n4:5434505 n4:4536096 n4:1947788 n4:8893012
n3:druhVysledku
n15:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
219038
n3:idVysledku
RIV/67985904:_____/11:00367748
n3:jazykVysledku
n10:eng
n3:klicovaSlova
miniature pig; mesenchymal stem cells; cell differentiation
n3:klicoveSlovo
n5:mesenchymal%20stem%20cells n5:miniature%20pig n5:cell%20differentiation
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[AECC19365437]
n3:nazevZdroje
Physiological Research
n3:obor
n17:EB
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n16:1M0538 n16:2B06130
n3:rokUplatneniVysledku
n7:2011
n3:svazekPeriodika
60
n3:tvurceVysledku
Juhásová, Jana Strnádel, Ján Klíma, Jiří Motlík, Jan Holubová, Monika Juhás, Štefan
n3:wos
000293138600017
n3:zamer
n6:AV0Z50450515
s:issn
0862-8408
s:numberOfPages
13