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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F11%3A00359480%21RIV12-AV0-67985904
rdf:type
n4:Vysledek skos:Concept
dcterms:description
Serum and plasma-based biomarker discovery requires technologies with specific capabilities: sufficient proteome coverage and depth; technical reproducibly; and the scalability to enable analysis on substantial volumes of a large number of samples at reasonable cost. We have shown that plasma samples processed using ProteomeLab IgY-12 LC10 Proteome Partitioning Kits to selectively remove the 12 most highly-abundant proteins, followed by intact protein separation by two-dimensional liquid chromatography (2DLC, chromatofocusing and reversed phase) can unique enrich for middle –lower-abundant proteins. Equally, 1DLC (reversed phase) separation of intact proteins is complementary to 2DLC. The serial use of a single piece of equipment can be prohibitively time-consuming and thus, this chapter also describes the harmonization of multiple LC instruments in order to minimize technical variation and ensure reproducibility. Serum and plasma-based biomarker discovery requires technologies with specific capabilities: sufficient proteome coverage and depth; technical reproducibly; and the scalability to enable analysis on substantial volumes of a large number of samples at reasonable cost. We have shown that plasma samples processed using ProteomeLab IgY-12 LC10 Proteome Partitioning Kits to selectively remove the 12 most highly-abundant proteins, followed by intact protein separation by two-dimensional liquid chromatography (2DLC, chromatofocusing and reversed phase) can unique enrich for middle –lower-abundant proteins. Equally, 1DLC (reversed phase) separation of intact proteins is complementary to 2DLC. The serial use of a single piece of equipment can be prohibitively time-consuming and thus, this chapter also describes the harmonization of multiple LC instruments in order to minimize technical variation and ensure reproducibility.
dcterms:title
Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma
skos:prefLabel
Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma
skos:notation
RIV/67985904:_____/11:00359480!RIV12-AV0-67985904
n4:predkladatel
n16:ico%3A67985904
n5:aktivita
n12:Z
n5:aktivity
Z(AV0Z50450515)
n5:dodaniDat
n10:2012
n5:domaciTvurceVysledku
n19:6541186
n5:druhVysledku
n20:C
n5:duvernostUdaju
n13:S
n5:entitaPredkladatele
n11:predkladatel
n5:idSjednocenehoVysledku
205103
n5:idVysledku
RIV/67985904:_____/11:00359480
n5:jazykVysledku
n18:eng
n5:klicovaSlova
liquid chromatography; chromatofocusing; reversed phase
n5:klicoveSlovo
n6:reversed%20phase n6:chromatofocusing n6:liquid%20chromatography
n5:kontrolniKodProRIV
[678B38224CCC]
n5:mistoVydani
NEW YORK
n5:nazevZdroje
Serum/Plasma Proteomics Metods and Protocols
n5:obor
n17:EB
n5:pocetDomacichTvurcuVysledku
1
n5:pocetStranKnihy
397
n5:pocetTvurcuVysledku
6
n5:rokUplatneniVysledku
n10:2011
n5:tvurceVysledku
Van Eyk, J. Sheng, S. Skalníková, Helena Fu, Q. Meng, A. Everett, A.
n5:zamer
n8:AV0Z50450515
s:numberOfPages
17
n15:hasPublisher
Humana Press
n9:isbn
978-1-61779-067-6