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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F10%3A00353420%21RIV12-AV0-67985904
rdf:type
skos:Concept n10:Vysledek
dcterms:description
Using PCR and inverse PCR techniques we obtained a 4,498 bp nucleotide sequence FN424076 encompassing the complete coding sequence of the porcine insulin receptor substrate 4 (IRS4) gene and its proximal promoter. The 1,269 amino acid porcine protein deduced from the nucleotide sequence shares 92% identity with the human IRS4 and possesses the same domains and the same number of tyrosine phosphorylation motifs as the human protein. We detected substitution FN424076:g.96C<G in the promoter region that segregates in Meishan and a synonymous substitution FN424076:g.1829T<C in the coding sequence with allele C present only in Meishan. Using PCR and inverse PCR techniques we obtained a 4,498 bp nucleotide sequence FN424076 encompassing the complete coding sequence of the porcine insulin receptor substrate 4 (IRS4) gene and its proximal promoter. The 1,269 amino acid porcine protein deduced from the nucleotide sequence shares 92% identity with the human IRS4 and possesses the same domains and the same number of tyrosine phosphorylation motifs as the human protein. We detected substitution FN424076:g.96C<G in the promoter region that segregates in Meishan and a synonymous substitution FN424076:g.1829T<C in the coding sequence with allele C present only in Meishan.
dcterms:title
Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study
skos:prefLabel
Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study
skos:notation
RIV/67985904:_____/10:00353420!RIV12-AV0-67985904
n3:aktivita
n12:Z n12:P
n3:aktivity
P(GA523/07/0353), P(GAP502/10/1216), Z(AV0Z50450515)
n3:cisloPeriodika
-
n3:dodaniDat
n5:2012
n3:domaciTvurceVysledku
n14:3380602 n14:2114038 n14:2529157
n3:druhVysledku
n18:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
279894
n3:idVysledku
RIV/67985904:_____/10:00353420
n3:jazykVysledku
n4:eng
n3:klicovaSlova
PCR cloning; Polymorphism; IRS4
n3:klicoveSlovo
n8:Polymorphism n8:PCR%20cloning n8:IRS4
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[9D5BE5087A10]
n3:nazevZdroje
Molecular Biology Reports
n3:obor
n19:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
8
n3:projekt
n13:GAP502%2F10%2F1216 n13:GA523%2F07%2F0353
n3:rokUplatneniVysledku
n5:2010
n3:svazekPeriodika
38
n3:tvurceVysledku
Knoll, Aleš Rohrer, G. A. Bartenschlager, H. Vykoukalová, Z. Deeb, N. Mileham, A. Masopust, Martin Čepica, Stanislav
n3:wos
000289257100050
n3:zamer
n17:AV0Z50450515
s:issn
0301-4851
s:numberOfPages
7
n15:doi
10.1007/s11033-010-0402-x