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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F10%3A00345859%21RIV11-GA0-67985904
rdf:type
skos:Concept n15:Vysledek
dcterms:description
A feeding study was performed to monitor the effect of chitosan intake on the fecal microbiota of ten healthy human subjects. Diversity of microflora was monitored during 8 weeks including 4 weeks of chitosan supplementations. Using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons and quantitative PCR method we revealed possible changes originating in the overall bacterial composition and also in the subpopulation of Bifidobacterium group. DGGE profiles displayed high complexity and individuality for each subject. Considerable variations in the composition of band patterns were observed among different persons. A raised level of fecal Bacteroides in response to chitosan intake was found in all samples. Bifidobacterium levels following chitosan intake increased or remain unchanged. Non-significant increase was, surprisingly, found in the numbers of butyrate-producing bacteria. A feeding study was performed to monitor the effect of chitosan intake on the fecal microbiota of ten healthy human subjects. Diversity of microflora was monitored during 8 weeks including 4 weeks of chitosan supplementations. Using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons and quantitative PCR method we revealed possible changes originating in the overall bacterial composition and also in the subpopulation of Bifidobacterium group. DGGE profiles displayed high complexity and individuality for each subject. Considerable variations in the composition of band patterns were observed among different persons. A raised level of fecal Bacteroides in response to chitosan intake was found in all samples. Bifidobacterium levels following chitosan intake increased or remain unchanged. Non-significant increase was, surprisingly, found in the numbers of butyrate-producing bacteria.
dcterms:title
PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans
skos:prefLabel
PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans
skos:notation
RIV/67985904:_____/10:00345859!RIV11-GA0-67985904
n3:aktivita
n13:P n13:Z
n3:aktivity
P(GA525/08/0803), Z(AV0Z50450515)
n3:cisloPeriodika
4
n3:dodaniDat
n11:2011
n3:domaciTvurceVysledku
n7:6921558 n7:2524937 n7:2853892 n7:7273029 n7:4989341
n3:druhVysledku
n12:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
278338
n3:idVysledku
RIV/67985904:_____/10:00345859
n3:jazykVysledku
n10:eng
n3:klicovaSlova
Chitosan; Microbial stability; Microflora
n3:klicoveSlovo
n9:Microflora n9:Microbial%20stability n9:Chitosan
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[BFC2D1CF5D03]
n3:nazevZdroje
Folia Microbiologica
n3:obor
n4:FB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n18:GA525%2F08%2F0803
n3:rokUplatneniVysledku
n11:2010
n3:svazekPeriodika
55
n3:tvurceVysledku
Šimůnek, Jiří Koppová, Ingrid Fliegerová, Kateřina Kopečný, Jan Mrázek, Jakub
n3:wos
000281277900012
n3:zamer
n6:AV0Z50450515
s:issn
0015-5632
s:numberOfPages
7