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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F09%3A00338301%21RIV10-MSM-67985904
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Proteomic profiling of cancer cells was carried out with a view to addressing leukaemia and its treatment with a perspective towards novel diagnostic and/or treatment modalities. Earlier protein changes that precede the onset of apoptosis were studied using proteomic analysis of cells treated with anticancer drug cisplatin. To reliably quantify maximum number of proteins, combination of three proteomic techniques were used, namely 2-D electrophoresis, 2-D liquid chromatography and SILAC approach. The combination of several different methods was able to reveal and identify a large number of different proteins of interest. With such an approach we aim to extend our understanding of the on-going’ changes in CEM cell proteome induced by cisplatin treatment. Proteomic profiling of cancer cells was carried out with a view to addressing leukaemia and its treatment with a perspective towards novel diagnostic and/or treatment modalities. Earlier protein changes that precede the onset of apoptosis were studied using proteomic analysis of cells treated with anticancer drug cisplatin. To reliably quantify maximum number of proteins, combination of three proteomic techniques were used, namely 2-D electrophoresis, 2-D liquid chromatography and SILAC approach. The combination of several different methods was able to reveal and identify a large number of different proteins of interest. With such an approach we aim to extend our understanding of the on-going’ changes in CEM cell proteome induced by cisplatin treatment.
dcterms:title
Proteome of leukaemic cells after cisplatin treatment - comparison of three independent analytical techniques Proteome of leukaemic cells after cisplatin treatment - comparison of three independent analytical techniques
skos:prefLabel
Proteome of leukaemic cells after cisplatin treatment - comparison of three independent analytical techniques Proteome of leukaemic cells after cisplatin treatment - comparison of three independent analytical techniques
skos:notation
RIV/67985904:_____/09:00338301!RIV10-MSM-67985904
n3:aktivita
n4:P n4:Z
n3:aktivity
P(LC07017), Z(AV0Z50200510), Z(AV0Z50450515)
n3:dodaniDat
n14:2010
n3:domaciTvurceVysledku
n18:1147633 n18:8749779 n18:2323877 n18:6541186
n3:druhVysledku
n15:D
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
337276
n3:idVysledku
RIV/67985904:_____/09:00338301
n3:jazykVysledku
n9:eng
n3:klicovaSlova
Proteomics; Cis-platin; Leukaemic cells
n3:klicoveSlovo
n13:Proteomics n13:Cis-platin n13:Leukaemic%20cells
n3:kontrolniKodProRIV
[E99232FA9D61]
n3:mistoKonaniAkce
Budapešť
n3:mistoVydani
Budapest
n3:nazevZdroje
3rd Central and Eastern Proteomics Conference, 2009
n3:obor
n20:FK
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
11
n3:projekt
n21:LC07017
n3:rokUplatneniVysledku
n14:2009
n3:tvurceVysledku
Hrabáková, Rita Kovářová, Hana Havlíček, Vladimír Skalníková, Helena Pompach, Petr Strohalm, Martin Hajdúch, M. Novák, Petr Man, Petr Džubák, P. Martinková, Jiřina
n3:typAkce
n12:EUR
n3:zahajeniAkce
2009-10-06+02:00
n3:zamer
n17:AV0Z50200510 n17:AV0Z50450515
s:numberOfPages
1
n7:hasPublisher
Hungarian proteomic society
n10:isbn
978-963-9319-99-8