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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F09%3A00337404%21RIV10-AV0-67985904
rdf:type
skos:Concept n6:Vysledek
dcterms:description
Regulation of gene expression at the translational level is particularly essential during developmental periods, when transcription is impaired. According to the closed-loop model of translational initiation, we have analyzed components of the 5'-mRNA cap-binding complex eIF4F (eIF4E, eIF4G, eIF4A), the eIF4E repressor 4E-BP1, and 3'-mRNA poly-(A) tail-associated proteins (PABP1 and 3, PAIP1 and 2, CPEB1, Maskin) during in vitro maturation of bovine oocytes and early embryonic development up to the 16-cell stage. Furthermore, we have elucidated the activity of distinct kinases which are potentially involved in their phosphorylation. Major phosphorylation of specific target sequences of PKA, PKB, PKC, CDKs, ATM/ATR, and MAPK were observed in M II stage oocytes. Furthermore, main changes in the abundance and/or phosphorylation of distinct mRNA-binding factors occur at the transition from M II stage oocytes to 2-cell embryos. In conclusion, the results indicate that, at the transition from oocyte to e... Regulation of gene expression at the translational level is particularly essential during developmental periods, when transcription is impaired. According to the closed-loop model of translational initiation, we have analyzed components of the 5'-mRNA cap-binding complex eIF4F (eIF4E, eIF4G, eIF4A), the eIF4E repressor 4E-BP1, and 3'-mRNA poly-(A) tail-associated proteins (PABP1 and 3, PAIP1 and 2, CPEB1, Maskin) during in vitro maturation of bovine oocytes and early embryonic development up to the 16-cell stage. Furthermore, we have elucidated the activity of distinct kinases which are potentially involved in their phosphorylation. Major phosphorylation of specific target sequences of PKA, PKB, PKC, CDKs, ATM/ATR, and MAPK were observed in M II stage oocytes. Furthermore, main changes in the abundance and/or phosphorylation of distinct mRNA-binding factors occur at the transition from M II stage oocytes to 2-cell embryos. In conclusion, the results indicate that, at the transition from oocyte to e...
dcterms:title
Translational regulation by mRNA associated factors during bovine oocyte maturation and early embryonic development Translational regulation by mRNA associated factors during bovine oocyte maturation and early embryonic development
skos:prefLabel
Translational regulation by mRNA associated factors during bovine oocyte maturation and early embryonic development Translational regulation by mRNA associated factors during bovine oocyte maturation and early embryonic development
skos:notation
RIV/67985904:_____/09:00337404!RIV10-AV0-67985904
n4:aktivita
n19:Z n19:P
n4:aktivity
P(GA524/07/1087), Z(AV0Z50450515)
n4:dodaniDat
n14:2010
n4:domaciTvurceVysledku
n16:1114921
n4:druhVysledku
n13:D
n4:duvernostUdaju
n18:S
n4:entitaPredkladatele
n20:predkladatel
n4:idSjednocenehoVysledku
346765
n4:idVysledku
RIV/67985904:_____/09:00337404
n4:jazykVysledku
n9:eng
n4:klicovaSlova
Oocyte maturation; Bovine embryo
n4:klicoveSlovo
n12:Oocyte%20maturation n12:Bovine%20embryo
n4:kontrolniKodProRIV
[E10B48D33B5A]
n4:mistoKonaniAkce
Leipzig
n4:mistoVydani
Malden
n4:nazevZdroje
Reproduction in domestic animals
n4:obor
n8:ED
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
5
n4:projekt
n15:GA524%2F07%2F1087
n4:rokUplatneniVysledku
n14:2009
n4:tvurceVysledku
Bhojawni, M. Tomek, W. Smiljakovic, T. Kubelka, Michal Siemer, C.
n4:typAkce
n11:WRD
n4:zahajeniAkce
2009-02-26+01:00
n4:zamer
n17:AV0Z50450515
s:issn
0936-6768
s:numberOfPages
2
n7:hasPublisher
Wiley-Blackwell