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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F09%3A00329884%21RIV10-AV0-67985904
rdf:type
n15:Vysledek skos:Concept
dcterms:description
The aim of this study was to investigate the involvement of the serine/threonine protein kinase AKT (also called protein kinase B) in the control of meiosis of porcine denuded oocytes (DOs) matured in vitro. Western blot analysis revealed that the two principal AKT phosphorylation sites, Ser473 and Thr308, are phosphorylated at different stages of meiosis. In freshly isolated germinal vesicle (GV)-stage DOs, Ser473 was already phosphorylated. After the onset of oocyte maturation, the intensity of the Ser473 phosphorylation increased, however, which declined sharply when DOs underwent GV breakdown (GVBD) and remained at low levels in metaphase I- and II-stage (MI- and MII-stage). In contrast, phosphorylation of Thr308 was increased by the time of GVBD and reached maximum at MI-stage. A peak of AKT activity was noticed around GVBD and activity of AKT declined at MI-stage. To assess the role of AKT during meiosis, porcine DOs were cultured in 50 mikroM SH-6, a specific The aim of this study was to investigate the involvement of the serine/threonine protein kinase AKT (also called protein kinase B) in the control of meiosis of porcine denuded oocytes (DOs) matured in vitro. Western blot analysis revealed that the two principal AKT phosphorylation sites, Ser473 and Thr308, are phosphorylated at different stages of meiosis. In freshly isolated germinal vesicle (GV)-stage DOs, Ser473 was already phosphorylated. After the onset of oocyte maturation, the intensity of the Ser473 phosphorylation increased, however, which declined sharply when DOs underwent GV breakdown (GVBD) and remained at low levels in metaphase I- and II-stage (MI- and MII-stage). In contrast, phosphorylation of Thr308 was increased by the time of GVBD and reached maximum at MI-stage. A peak of AKT activity was noticed around GVBD and activity of AKT declined at MI-stage. To assess the role of AKT during meiosis, porcine DOs were cultured in 50 mikroM SH-6, a specific
dcterms:title
AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes
skos:prefLabel
AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes AKT (protein kinase B) is implicated in meiotic maturation of porcine oocytes
skos:notation
RIV/67985904:_____/09:00329884!RIV10-AV0-67985904
n3:aktivita
n6:Z n6:P
n3:aktivity
P(GA204/06/1297), P(GA523/03/0857), P(GA524/07/1087), Z(AV0Z50450515)
n3:cisloPeriodika
-
n3:dodaniDat
n13:2010
n3:domaciTvurceVysledku
n11:6275532 n11:3887103 n11:5434505 n11:8523487 n11:1114921
n3:druhVysledku
n12:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
302268
n3:idVysledku
RIV/67985904:_____/09:00329884
n3:jazykVysledku
n16:eng
n3:klicovaSlova
protein kinase; porcine oocyte; oocyte maturation
n3:klicoveSlovo
n4:protein%20kinase n4:porcine%20oocyte n4:oocyte%20maturation
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[665DBA6DB207]
n3:nazevZdroje
Reproduction
n3:obor
n18:CE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n9:GA524%2F07%2F1087 n9:GA523%2F03%2F0857 n9:GA204%2F06%2F1297
n3:rokUplatneniVysledku
n13:2009
n3:svazekPeriodika
138
n3:tvurceVysledku
Kubelka, Michal Šušor, Andrej Motlík, Jan Šolc, Petr Kalous, Jaroslav
n3:wos
000270806100004
n3:zamer
n14:AV0Z50450515
s:issn
1470-1626
s:numberOfPages
10