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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F08%3A00314274%21RIV09-AV0-67985904
rdf:type
n6:Vysledek skos:Concept
dcterms:description
To determine the organization of transferrin (TF) locus in the Salmo trutta genome, partial DNA and cDNA sequencing, fluorescent in situ hybridization (FISH) and Salmo salar BAC analysis were performed. The real-time PCR data showed that each of the two TF types (TF1 and TF2) existed in one copy only and that the transcription of TF2 was considerably lower compared with TF1. Using FISH, hybridization signals were observed on two medium-sized acrocentric chromosomes of S. trutta karyotype. A TF type-specific PCR followed by a restriction analysis revealed the presence of two TF loci in the majority of analysed BAC clones. It was concluded that the TF gene is duplicated in the genome of S. trutta, and that the two TF loci are located adjacent to one another on the same chromosome. The differing transcription levels of TF1 and TF2 appear to depend on the corresponding promoter activity, which at least for TF2 seems to vary between different Salmo congeners. Organizace genu pro transferin v genomu Salmu trutta byla studpvána pomocí DNA a cDNA sekvenování, in situ hybridizací (FISH) a BAC knihovnami z genomu Salmo salar. RT-PCR ukázala, že každá z obou typu TF existuje jen v jedné kopii a transkripční aktivita druhé je daleko nižší. Fyzická lokalizace TF lokusů pomocí FISH zjistila jejich místo v pericentromerické oblasti ne jednom páru středně velkých akrocentrických chromozómů. Analýza ukázala, ze TF lokus je duplikován a obě kopie leží tandemově za sebou na tom samém chromozómu. To determine the organization of transferrin (TF) locus in the Salmo trutta genome, partial DNA and cDNA sequencing, fluorescent in situ hybridization (FISH) and Salmo salar BAC analysis were performed. The real-time PCR data showed that each of the two TF types (TF1 and TF2) existed in one copy only and that the transcription of TF2 was considerably lower compared with TF1. Using FISH, hybridization signals were observed on two medium-sized acrocentric chromosomes of S. trutta karyotype. A TF type-specific PCR followed by a restriction analysis revealed the presence of two TF loci in the majority of analysed BAC clones. It was concluded that the TF gene is duplicated in the genome of S. trutta, and that the two TF loci are located adjacent to one another on the same chromosome. The differing transcription levels of TF1 and TF2 appear to depend on the corresponding promoter activity, which at least for TF2 seems to vary between different Salmo congeners.
dcterms:title
Důkaz dvou lokusů pro transferin v genomu Salmo trutta Evidence for two transferrin loci in the Salmo trutta genome Evidence for two transferrin loci in the Salmo trutta genome
skos:prefLabel
Evidence for two transferrin loci in the Salmo trutta genome Důkaz dvou lokusů pro transferin v genomu Salmo trutta Evidence for two transferrin loci in the Salmo trutta genome
skos:notation
RIV/67985904:_____/08:00314274!RIV09-AV0-67985904
n3:aktivita
n5:Z
n3:aktivity
Z(AV0Z50450515)
n3:cisloPeriodika
-
n3:dodaniDat
n13:2009
n3:domaciTvurceVysledku
n4:9818863
n3:druhVysledku
n17:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
366903
n3:idVysledku
RIV/67985904:_____/08:00314274
n3:jazykVysledku
n15:eng
n3:klicovaSlova
chromosomes; fluorescent in situ hybridization; real-time PCR
n3:klicoveSlovo
n12:fluorescent%20in%20situ%20hybridization n12:chromosomes n12:real-time%20PCR
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[AE02541B4DDC]
n3:nazevZdroje
Animal Genetics
n3:obor
n16:EG
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
7
n3:rokUplatneniVysledku
n13:2008
n3:svazekPeriodika
39
n3:tvurceVysledku
Snoj, A. Ráb, Petr Marič, S. Erjavec-Škerget, A. Kokalj-Vokač, N. Rozman, T. Dovc, P.
n3:wos
000261051800001
n3:zamer
n11:AV0Z50450515
s:issn
0268-9146
s:numberOfPages
9