This HTML5 document contains 44 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n7http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n17http://linked.opendata.cz/ontology/domain/vavai/
n11http://linked.opendata.cz/resource/domain/vavai/zamer/
n14http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F67985904%3A_____%2F08%3A00312643%21RIV09-AV0-67985904/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n9http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n15http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n13http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n8http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n16http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n10http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F08%3A00312643%21RIV09-AV0-67985904
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Chitinolytický systém anaerobních polycentrických bachorových hub rodu Orpinomyces a Anaeromyces byl studován ve třech frakcích- extracelulární, cytosolické frakce a frakce z buněčných stěn. Endochitinasa představovala dominantní enzym s nejvyšší aktivitou v cytosolické frakci. Endochitinasa u obou rodů byla stabilní v rozmezí pH 4,5 až 7 s optimálním pH 6,5. Endochitinasa u Orpinomyces byla stabilní do 50°C s optimální teplotou 50°C. Endochitinasa z Anaeromyces byla stabilní do 40°C s optimální teplotou 40°C. Nejvhodnějším substrátem pro endochitinasu u obou rodů byl chitin izolovaný z houbových buněčných stěn. Enzymová aktivita obou endochitinas byla inhibována iontem Hg2+ a aktivována iontem Mg2+. Obě endochitinasy byli inhibovány 10 mM SDS a aktivovány iodoacetamidem. Chitinolytic system of anaerobic polycentric rumen fungi of genera Orpinomyces and Anaeromyces was studied in three crude enzyme fractions- extracellular, cytosolic and cell wall fraction. Endochitinase was found as a dominant enzyme with highest activity in cytosolic fraction. Endochitinases of both genera were stable from pH 4.5 to 7 with optimal pH 6.5. Orpinomyces endochitinase was stable up to 50 0C with optimal temperature 50 0C. Anaeromyces endochitinase was stable up to 40 0C with optimal temperature also 40 0C. The most suitable substrate for both endochitinases was fungal cell wall chitin. Enzyme activities were inhibited by Hg2+ ions and activated by Mg2+ ions. Both endochitinases were inhibited by 10 mM SDS and activated by iodacetamide. Chitinolytic system of anaerobic polycentric rumen fungi of genera Orpinomyces and Anaeromyces was studied in three crude enzyme fractions- extracellular, cytosolic and cell wall fraction. Endochitinase was found as a dominant enzyme with highest activity in cytosolic fraction. Endochitinases of both genera were stable from pH 4.5 to 7 with optimal pH 6.5. Orpinomyces endochitinase was stable up to 50 0C with optimal temperature 50 0C. Anaeromyces endochitinase was stable up to 40 0C with optimal temperature also 40 0C. The most suitable substrate for both endochitinases was fungal cell wall chitin. Enzyme activities were inhibited by Hg2+ ions and activated by Mg2+ ions. Both endochitinases were inhibited by 10 mM SDS and activated by iodacetamide.
dcterms:title
Characterization of chitinases of polycentric anaerobic rumen fungi Charakterizace chitinas polycentrických anaerobních bachorových hub Characterization of chitinases of polycentric anaerobic rumen fungi
skos:prefLabel
Characterization of chitinases of polycentric anaerobic rumen fungi Charakterizace chitinas polycentrických anaerobních bachorových hub Characterization of chitinases of polycentric anaerobic rumen fungi
skos:notation
RIV/67985904:_____/08:00312643!RIV09-AV0-67985904
n4:aktivita
n13:Z
n4:aktivity
Z(AV0Z50450515)
n4:cisloPeriodika
3
n4:dodaniDat
n10:2009
n4:domaciTvurceVysledku
n7:4989341 n7:7195176 n7:7273029
n4:druhVysledku
n16:J
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
359578
n4:idVysledku
RIV/67985904:_____/08:00312643
n4:jazykVysledku
n8:eng
n4:klicovaSlova
endochitinase; anaerobic; rumen fungi
n4:klicoveSlovo
n9:endochitinase n9:anaerobic n9:rumen%20fungi
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[1C286AB424B3]
n4:nazevZdroje
Folia Microbiologica
n4:obor
n12:EE
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
3
n4:rokUplatneniVysledku
n10:2008
n4:svazekPeriodika
53
n4:tvurceVysledku
Šimůnek, Jiří Fliegerová, Kateřina Novotná, Zuzana
n4:wos
000257941200014
n4:zamer
n11:AV0Z50450515
s:issn
0015-5632
s:numberOfPages
5