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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F02%3A21023078%21RIV%2F2003%2FAV0%2FA21003%2FN
rdf:type
skos:Concept n15:Vysledek
dcterms:description
A strictly anaerobic, mesophilic and chitinolytic bacterial strain was isolated from human feces. Based on morphological and physiological properties and 16S rRNA sequence analysis the strain was identified as Clostridium paraputrificum.The strain utilized chitin and N-acetyl-D-glucosamine, grew on glucose and hydrolyzed starch. Cultivation of the strain with colloidal chitin as the growth substrate resulted in the production of gas and formation of acetate and lactate and only small quantities of propionate and butyrate. In the course of a 10-d cultivation with chitin, the endochitinase activity was detected after 1 d and gradually increased, reaching maximum after 3 d. The beta-N-acetylglucosaminidase activity appeared just at the beginning of the cultivation, increased to day 2 and then remained nearly constant. More than 90 of chitin added was degraded within 2 d of cultivation. On the zymogram of the extracelular chitinolytic complex were visible at least 6 isoenzymes with molar mass 43.5-65.0 k A strictly anaerobic, mesophilic and chitinolytic bacterial strain was isolated from human feces. Based on morphological and physiological properties and 16S rRNA sequence analysis the strain was identified as Clostridium paraputrificum.The strain utilized chitin and N-acetyl-D-glucosamine, grew on glucose and hydrolyzed starch. Cultivation of the strain with colloidal chitin as the growth substrate resulted in the production of gas and formation of acetate and lactate and only small quantities of propionate and butyrate. In the course of a 10-d cultivation with chitin, the endochitinase activity was detected after 1 d and gradually increased, reaching maximum after 3 d. The beta-N-acetylglucosaminidase activity appeared just at the beginning of the cultivation, increased to day 2 and then remained nearly constant. More than 90 of chitin added was degraded within 2 d of cultivation. On the zymogram of the extracelular chitinolytic complex were visible at least 6 isoenzymes with molar mass 43.5-65.0 k
dcterms:title
Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract. Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract.
skos:prefLabel
Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract. Identification and Characterization of Clostridium paraputrificum, a Chitinolytic Bacterium of Human Digestive Tract.
skos:notation
RIV/67985904:_____/02:21023078!RIV/2003/AV0/A21003/N
n3:strany
559;564
n3:aktivita
n6:P n6:Z
n3:aktivity
P(GA525/00/0984), P(KSK5052113), Z(AV0Z5045916)
n3:cisloPeriodika
5
n3:dodaniDat
n5:2003
n3:domaciTvurceVysledku
n13:6921558 n13:1478966 n13:2457067 n13:4989341
n3:druhVysledku
n7:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
648265
n3:idVysledku
RIV/67985904:_____/02:21023078
n3:jazykVysledku
n16:eng
n3:klicovaSlova
Clostridium paraputrificum; Chitinolytic bacterium; digestive tract
n3:klicoveSlovo
n12:digestive%20tract n12:Clostridium%20paraputrificum n12:Chitinolytic%20bacterium
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[518E827B3008]
n3:nazevZdroje
Folia Microbiologica
n3:obor
n17:EE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n8:KSK5052113 n8:GA525%2F00%2F0984
n3:rokUplatneniVysledku
n5:2002
n3:svazekPeriodika
47
n3:tvurceVysledku
Kopečný, Jan Hodrová, Blanka Šimůnek, Jiří Bartoňová, Hana
n3:zamer
n18:AV0Z5045916
s:issn
0015-5632
s:numberOfPages
5