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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F00%3A21003030%21RIV%2F2003%2FAV0%2FA21003%2FN
rdf:type
skos:Concept n17:Vysledek
dcterms:description
M-phase synchronized bovine blastomeres were used to study the effect of nuclear-cytoplasmic synchronization on the developmental potential after nuclear transfer (NT). The capacity of nocodazole and benomyl to reversibly synchronize blastomeres from embryos in M-phase was evaluated. Nocodazole reversibly arrested bovine embryos at the studied stages and induced high rates of M-phases in morulae and compact morulae. In contrast, benomyl was less efficient than nocodazole to synchronize in M-phase. After transfer of an M-phase blastomere, premature chromatin condensation was the prevalent finding 1 hr post-fusion (hpf). Condensed chromosomes non-arranged in the equatorial plate (1-3 hpf) that acquired an organized structure over time (3-7 hpf) were subsequently observed. Anaphase;telophase structures were predominantly recorded at 4-9 hpf. About 50% of the embryos activated at both 3-4 and 6-7 hpf extruded a polar body-like structure 5 hr after activation, but this was not observed in embryos activated M-phase synchronized bovine blastomeres were used to study the effect of nuclear-cytoplasmic synchronization on the developmental potential after nuclear transfer (NT). The capacity of nocodazole and benomyl to reversibly synchronize blastomeres from embryos in M-phase was evaluated. Nocodazole reversibly arrested bovine embryos at the studied stages and induced high rates of M-phases in morulae and compact morulae. In contrast, benomyl was less efficient than nocodazole to synchronize in M-phase. After transfer of an M-phase blastomere, premature chromatin condensation was the prevalent finding 1 hr post-fusion (hpf). Condensed chromosomes non-arranged in the equatorial plate (1-3 hpf) that acquired an organized structure over time (3-7 hpf) were subsequently observed. Anaphase;telophase structures were predominantly recorded at 4-9 hpf. About 50% of the embryos activated at both 3-4 and 6-7 hpf extruded a polar body-like structure 5 hr after activation, but this was not observed in embryos activated
dcterms:title
Behavior of M-phase synchronized blastomeres after nuclear transfer in cattle. Behavior of M-phase synchronized blastomeres after nuclear transfer in cattle.
skos:prefLabel
Behavior of M-phase synchronized blastomeres after nuclear transfer in cattle. Behavior of M-phase synchronized blastomeres after nuclear transfer in cattle.
skos:notation
RIV/67985904:_____/00:21003030!RIV/2003/AV0/A21003/N
n3:strany
37;47
n3:aktivita
n4:Z n4:P
n3:aktivity
P(KSK2038602), Z(AV0Z5045916)
n3:cisloPeriodika
1
n3:dodaniDat
n6:2003
n3:domaciTvurceVysledku
n12:5434505
n3:druhVysledku
n9:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
705493
n3:idVysledku
RIV/67985904:_____/00:21003030
n3:jazykVysledku
n15:eng
n3:klicovaSlova
N/A
n3:klicoveSlovo
n16:N%2FA
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[391D023C2840]
n3:nazevZdroje
Molecular Reproduction and Development
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n18:KSK2038602
n3:rokUplatneniVysledku
n6:2000
n3:svazekPeriodika
57
n3:tvurceVysledku
Stojkovic, M. Motlík, Jan Alberio, R. Wolf, E. Zakhartchenko, V.
n3:zamer
n14:AV0Z5045916
s:issn
1040-452X
s:numberOfPages
11