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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F00%3A21003029%21RIV%2F2003%2FAV0%2FA21003%2FN
rdf:type
n14:Vysledek skos:Concept
dcterms:description
Activation of bovine oocytes by experimental procedures that closely mimic normal fertilization and allow to obtain haploid oocytes is essential both for intracytoplasmic sperm injection (ICSI) and for nuclear transfer. Therefore, with the goal of producing haploid activated oocytes, this study evaluated whether bohemine, either alone or in combination with ionomycin, is able to activate young matured bovine oocytes. Furthermore, the effect of bohemine on the patterns of DNA synthesis after pronuclear formation as well as changes in his tone H1 kinase and MAP kinase activities during the process of activation were studied. Our results with bohemine show that the specific inhibition of CDKs in metaphase II bovine oocytes induces parthenogenetic activation in a dose-dependent manner (25, 50, and 100 mu M, respectively), either alone (3%, 30%, and 50%) or in combination with ionomycin (30%, 70%, and 87.5%). A single pronucleus and extrusion of the second polar body was observed (97%) when Ca2+ influx was Activation of bovine oocytes by experimental procedures that closely mimic normal fertilization and allow to obtain haploid oocytes is essential both for intracytoplasmic sperm injection (ICSI) and for nuclear transfer. Therefore, with the goal of producing haploid activated oocytes, this study evaluated whether bohemine, either alone or in combination with ionomycin, is able to activate young matured bovine oocytes. Furthermore, the effect of bohemine on the patterns of DNA synthesis after pronuclear formation as well as changes in his tone H1 kinase and MAP kinase activities during the process of activation were studied. Our results with bohemine show that the specific inhibition of CDKs in metaphase II bovine oocytes induces parthenogenetic activation in a dose-dependent manner (25, 50, and 100 mu M, respectively), either alone (3%, 30%, and 50%) or in combination with ionomycin (30%, 70%, and 87.5%). A single pronucleus and extrusion of the second polar body was observed (97%) when Ca2+ influx was
dcterms:title
Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases. Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases.
skos:prefLabel
Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases. Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases.
skos:notation
RIV/67985904:_____/00:21003029!RIV/2003/AV0/A21003/N
n3:strany
422;432
n3:aktivita
n8:P n8:Z
n3:aktivity
P(KSK2038602), Z(AV0Z5045916)
n3:cisloPeriodika
4
n3:dodaniDat
n11:2003
n3:domaciTvurceVysledku
n15:5434505 n15:1114921
n3:druhVysledku
n10:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
703878
n3:idVysledku
RIV/67985904:_____/00:21003029
n3:jazykVysledku
n9:eng
n3:klicovaSlova
N/A
n3:klicoveSlovo
n17:N%2FA
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[E0D2AAE60364]
n3:nazevZdroje
Molecular Reproduction and Development
n3:obor
n4:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n12:KSK2038602
n3:rokUplatneniVysledku
n11:2000
n3:svazekPeriodika
55
n3:tvurceVysledku
Kubelka, Michal Motlík, Jan Alberio, R. Hajduch, M. Zakhartchenko, V. Wolf, E.
n3:zamer
n16:AV0Z5045916
s:issn
1040-452X
s:numberOfPages
11