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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F00%3A21003012%21RIV%2F2003%2FAV0%2FA21003%2FN
rdf:type
n12:Vysledek skos:Concept
dcterms:description
Bovine oocytes originated from follicles of two different size categories (medium (M), 3-6mm and small (S), 1-2mm) were cultured for 24 and 70h, respectively, in a meiosis-inhibiting medium (MIM) supplemented with 100&mgr;M butyrolactone I (BL I). At the end of culture, cumulus oocyte complexes (COC) from M and S follicles were labeled by 3H-uridine for 30min. The autoradiography (ARG) of semi-thin sections of COC showed the labeling of the germinal vesicles (GV) as: (1) The COC of the M category labeled immediately after isolation from follicles showed only weak labeling (+) of the GV. The COC of the S category labeled immediately after their isolation showed mostly intensive labeling (++++, +++) of the GV. (2) When the COC were labeled after 24 and 70h of culture in MIM, no labeling was observed in the M category. The S category of oocytes showed the slightly decreased labeling (+++, ++) after 24h and negligible labeling after 70h of culture. The pattern of very intensive labeling of granulosa cell Bovine oocytes originated from follicles of two different size categories (medium (M), 3-6mm and small (S), 1-2mm) were cultured for 24 and 70h, respectively, in a meiosis-inhibiting medium (MIM) supplemented with 100&mgr;M butyrolactone I (BL I). At the end of culture, cumulus oocyte complexes (COC) from M and S follicles were labeled by 3H-uridine for 30min. The autoradiography (ARG) of semi-thin sections of COC showed the labeling of the germinal vesicles (GV) as: (1) The COC of the M category labeled immediately after isolation from follicles showed only weak labeling (+) of the GV. The COC of the S category labeled immediately after their isolation showed mostly intensive labeling (++++, +++) of the GV. (2) When the COC were labeled after 24 and 70h of culture in MIM, no labeling was observed in the M category. The S category of oocytes showed the slightly decreased labeling (+++, ++) after 24h and negligible labeling after 70h of culture. The pattern of very intensive labeling of granulosa cell
dcterms:title
Culture of bovine oocytes from small antral follicles in meiosis-inhibiting medium with butyrolactone I: RNA synthesis, nucleolar morphology and meiotic competence. Culture of bovine oocytes from small antral follicles in meiosis-inhibiting medium with butyrolactone I: RNA synthesis, nucleolar morphology and meiotic competence.
skos:prefLabel
Culture of bovine oocytes from small antral follicles in meiosis-inhibiting medium with butyrolactone I: RNA synthesis, nucleolar morphology and meiotic competence. Culture of bovine oocytes from small antral follicles in meiosis-inhibiting medium with butyrolactone I: RNA synthesis, nucleolar morphology and meiotic competence.
skos:notation
RIV/67985904:_____/00:21003012!RIV/2003/AV0/A21003/N
n3:strany
1;111
n3:aktivita
n4:P n4:Z
n3:aktivity
P(GV524/96/K162), P(KSK2038602), Z(AV0Z5045916)
n3:cisloPeriodika
1-2
n3:dodaniDat
n13:2003
n3:domaciTvurceVysledku
n14:5196280 n14:8905029 n14:7205120 n14:5434505
n3:druhVysledku
n18:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
707810
n3:idVysledku
RIV/67985904:_____/00:21003012
n3:jazykVysledku
n9:eng
n3:klicovaSlova
N/A
n3:klicoveSlovo
n11:N%2FA
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[88E3B913D664]
n3:nazevZdroje
Animal Reproduction Science
n3:obor
n7:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n10:GV524%2F96%2FK162 n10:KSK2038602
n3:rokUplatneniVysledku
n13:2000
n3:svazekPeriodika
64
n3:tvurceVysledku
Vodička, Petr Pavlok, Antonín Kaňka, Jiří Motlík, Jan
n3:zamer
n8:AV0Z5045916
s:issn
0378-4320
s:numberOfPages
11