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Statements

Subject Item
n2:RIV%2F67985904%3A_____%2F00%3A21003009%21RIV%2F2003%2FAV0%2FA21003%2FN
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Proper conditions for the successful induction of a functional two step in vitro culture system for agricultural or clinical applications are hereby described in form of a two-step protocol. The first inhibitory culture of oocytes in media supplemented with specific cdk inhibitors (roscovitine, BL I) appears essential to promote appropriate oocyte-cumulus cell interactions and ensure production of ova competent to undergo normal embryogenesis. This inhibitory period should last for several days, depending on the origin of the follicles, and the degree of accumulation and storage of mRNAs and proteins essential for the acquisition of developmental competence in the oocytes. The full reversibility of the inhibitory step secures that during the second maturation step, oocytes reach metaphase IT with correctly arranged microtubuli in the meiotic spindle and well-expanded cumuli. Proper conditions for the successful induction of a functional two step in vitro culture system for agricultural or clinical applications are hereby described in form of a two-step protocol. The first inhibitory culture of oocytes in media supplemented with specific cdk inhibitors (roscovitine, BL I) appears essential to promote appropriate oocyte-cumulus cell interactions and ensure production of ova competent to undergo normal embryogenesis. This inhibitory period should last for several days, depending on the origin of the follicles, and the degree of accumulation and storage of mRNAs and proteins essential for the acquisition of developmental competence in the oocytes. The full reversibility of the inhibitory step secures that during the second maturation step, oocytes reach metaphase IT with correctly arranged microtubuli in the meiotic spindle and well-expanded cumuli.
dcterms:title
Impact of Two-step In Vitro Culture Systems on Developmental Potency of Oocytes. Impact of Two-step In Vitro Culture Systems on Developmental Potency of Oocytes.
skos:prefLabel
Impact of Two-step In Vitro Culture Systems on Developmental Potency of Oocytes. Impact of Two-step In Vitro Culture Systems on Developmental Potency of Oocytes.
skos:notation
RIV/67985904:_____/00:21003009!RIV/2003/AV0/A21003/N
n3:strany
267;271
n3:aktivita
n13:P n13:Z
n3:aktivity
P(GV524/96/K162), P(KSK2038602), Z(AV0Z5045916)
n3:cisloPeriodika
N/A
n3:dodaniDat
n11:2003
n3:domaciTvurceVysledku
n10:1114921 n10:4249909 n10:7205120 n10:5434505
n3:druhVysledku
n9:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
713208
n3:idVysledku
RIV/67985904:_____/00:21003009
n3:jazykVysledku
n4:eng
n3:klicovaSlova
N/A
n3:klicoveSlovo
n16:N%2FA
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[EF0CD276016E]
n3:nazevZdroje
Reproduction in Domestic Animals
n3:obor
n12:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n8:GV524%2F96%2FK162 n8:KSK2038602
n3:rokUplatneniVysledku
n11:2000
n3:svazekPeriodika
35
n3:tvurceVysledku
Lapathitis, Georgios Kubelka, Michal Pavlok, Antonín Motlík, Jan
n3:zamer
n6:AV0Z5045916
s:issn
0936-6768
s:numberOfPages
5