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Statements

Subject Item
n2:RIV%2F67985882%3A_____%2F14%3A00396975%21RIV15-GA0-67985882
rdf:type
n10:Vysledek skos:Concept
dcterms:description
The non-specific binding of non-target species to functionalized surfaces of biosensors continues to be challenge for biosensing in real-world media. Three different low-fouling and functionalizable surface platforms were employed to study the effect of functionalization on fouling resistance from several types of undiluted media including blood plasma and food media. The surface platforms investigated in this work included two polymer brushes: hydroxy-functional poly(2-hydroxyethyl methacrylate) (pHEMA) and carboxy-functional poly(carboxybetaine acrylamide) (pCBAA), and a standard OEG-based carboxy-functional alkanethiolate self-assembled monolayer (AT-SAM). The wet and dry polymer brushes were analyzed by AFM, ellipsometry, FT-IRRAS, and surface plasmon resonance (SPR). The surfaces were functionalized by the covalent attachment of antibodies, streptavidin, and oligonucleotides and the binding and biorecognition characteristics of the coatings were compared. We found that functionalization did not substantially affect the ultra-low fouling properties of pCBAA (plasma fouling of 20ng/cm2), a finding in contrast with pHEMA that completely lost its resistance to fouling after the activation of hydroxyl groups. Blocking a functionalized AT-SAM covalently with BSA decreased fouling down to the level comparable to unblocked pCBAA. However, the biorecognition capability of blocked functionalized AT-SAM was poor in comparison with functionalized pCBAA. Limits of detection of Escherichia coli O157:H7 in undiluted milk were determined to be 6104, 8105, and 6105cells/ml for pCBAA, pHEMA, and AT-SAM-blocked, respectively. Effect of analyte size on biorecognition activity of functionalized coatings was investigated and it was shown that the best performance in terms of overall fouling resistance and biorecognition capability is provided by pCBAA The non-specific binding of non-target species to functionalized surfaces of biosensors continues to be challenge for biosensing in real-world media. Three different low-fouling and functionalizable surface platforms were employed to study the effect of functionalization on fouling resistance from several types of undiluted media including blood plasma and food media. The surface platforms investigated in this work included two polymer brushes: hydroxy-functional poly(2-hydroxyethyl methacrylate) (pHEMA) and carboxy-functional poly(carboxybetaine acrylamide) (pCBAA), and a standard OEG-based carboxy-functional alkanethiolate self-assembled monolayer (AT-SAM). The wet and dry polymer brushes were analyzed by AFM, ellipsometry, FT-IRRAS, and surface plasmon resonance (SPR). The surfaces were functionalized by the covalent attachment of antibodies, streptavidin, and oligonucleotides and the binding and biorecognition characteristics of the coatings were compared. We found that functionalization did not substantially affect the ultra-low fouling properties of pCBAA (plasma fouling of 20ng/cm2), a finding in contrast with pHEMA that completely lost its resistance to fouling after the activation of hydroxyl groups. Blocking a functionalized AT-SAM covalently with BSA decreased fouling down to the level comparable to unblocked pCBAA. However, the biorecognition capability of blocked functionalized AT-SAM was poor in comparison with functionalized pCBAA. Limits of detection of Escherichia coli O157:H7 in undiluted milk were determined to be 6104, 8105, and 6105cells/ml for pCBAA, pHEMA, and AT-SAM-blocked, respectively. Effect of analyte size on biorecognition activity of functionalized coatings was investigated and it was shown that the best performance in terms of overall fouling resistance and biorecognition capability is provided by pCBAA
dcterms:title
Functionalized ultra-low fouling carboxy- and hydroxy-functional surface platforms: Functionalization capacity, biorecognition capability and resistance to fouling from undiluted biological media Functionalized ultra-low fouling carboxy- and hydroxy-functional surface platforms: Functionalization capacity, biorecognition capability and resistance to fouling from undiluted biological media
skos:prefLabel
Functionalized ultra-low fouling carboxy- and hydroxy-functional surface platforms: Functionalization capacity, biorecognition capability and resistance to fouling from undiluted biological media Functionalized ultra-low fouling carboxy- and hydroxy-functional surface platforms: Functionalization capacity, biorecognition capability and resistance to fouling from undiluted biological media
skos:notation
RIV/67985882:_____/14:00396975!RIV15-GA0-67985882
n4:aktivita
n11:I n11:P
n4:aktivity
I, P(EE2.3.30.0029), P(GBP205/12/G118)
n4:cisloPeriodika
15 January
n4:dodaniDat
n13:2015
n4:domaciTvurceVysledku
n5:4542355 n5:5874599 n5:5151821 n5:5270472 n5:6089976 n5:9632689
n4:druhVysledku
n9:J
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
17682
n4:idVysledku
RIV/67985882:_____/14:00396975
n4:jazykVysledku
n6:eng
n4:klicovaSlova
Low-fouling coatings; Polymer brushes; Surface chemistry
n4:klicoveSlovo
n14:Low-fouling%20coatings n14:Surface%20chemistry n14:Polymer%20brushes
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[080D2EF43DD2]
n4:nazevZdroje
Biosensors and Bioelectronics
n4:obor
n8:JA
n4:pocetDomacichTvurcuVysledku
6
n4:pocetTvurcuVysledku
11
n4:projekt
n17:GBP205%2F12%2FG118 n17:EE2.3.30.0029
n4:rokUplatneniVysledku
n13:2014
n4:svazekPeriodika
51
n4:tvurceVysledku
de los Santos Pereira, Andres Homola, Jiří Hegnerová, Kateřina Rodriguez-Emmenegger, Cesar Adam, Pavel Vaisocherová, Hana Riedel, Tomáš Ševců, Veronika Brynda, Eduard Špačková, Barbora Houska, Milan
n4:wos
000326905500021
s:issn
0956-5663
s:numberOfPages
8
n7:doi
10.1016/j.bios.2013.07.015