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Statements

Subject Item
n2:RIV%2F67985882%3A_____%2F13%3A00396519%21RIV14-GA0-67985882
rdf:type
n7:Vysledek skos:Concept
dcterms:description
A new concept of compact biochip for surface plasmon-enhanced fluorescence assays is reported. It takes advantage of the amplification of fluorescence signal through the coupling of fluorophore labels with confined and strongly enhanced field intensity of surface plasmons. In order to efficiently excite and collect the emitted fluorescence light via surface plasmons on a metallic sensor surface, (reverse) Kretschmann configuration is combined with diffractive optical elements embedded on the chip surface. These include a concentric relief grating for the imaging of highly directional surface plasmon-coupled emission to a detector. Additional linear grating is used for the generating of surface plasmons at the excitation wavelength on the sensor surface in order to increase the fluorescence excitation rate. The reported approach offers the increased intensity of fluorescence signal, reduced background, and compatibility with nanoimprint lithography for cost-effective preparation of sensor chip. The presented approach was implemented for biosensing in a model immunoassay experiment in which the limit of detection of 11 pM was achieved. A new concept of compact biochip for surface plasmon-enhanced fluorescence assays is reported. It takes advantage of the amplification of fluorescence signal through the coupling of fluorophore labels with confined and strongly enhanced field intensity of surface plasmons. In order to efficiently excite and collect the emitted fluorescence light via surface plasmons on a metallic sensor surface, (reverse) Kretschmann configuration is combined with diffractive optical elements embedded on the chip surface. These include a concentric relief grating for the imaging of highly directional surface plasmon-coupled emission to a detector. Additional linear grating is used for the generating of surface plasmons at the excitation wavelength on the sensor surface in order to increase the fluorescence excitation rate. The reported approach offers the increased intensity of fluorescence signal, reduced background, and compatibility with nanoimprint lithography for cost-effective preparation of sensor chip. The presented approach was implemented for biosensing in a model immunoassay experiment in which the limit of detection of 11 pM was achieved.
dcterms:title
Compact surface plasmon-enhanced fluorescence biochip Compact surface plasmon-enhanced fluorescence biochip
skos:prefLabel
Compact surface plasmon-enhanced fluorescence biochip Compact surface plasmon-enhanced fluorescence biochip
skos:notation
RIV/67985882:_____/13:00396519!RIV14-GA0-67985882
n7:predkladatel
n8:ico%3A67985882
n3:aktivita
n6:I n6:P
n3:aktivity
I, P(GBP205/12/G118)
n3:cisloPeriodika
8
n3:dodaniDat
n5:2014
n3:domaciTvurceVysledku
n9:6624634 n9:5151821 n9:9632689
n3:druhVysledku
n10:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
66054
n3:idVysledku
RIV/67985882:_____/13:00396519
n3:jazykVysledku
n12:eng
n3:klicovaSlova
Surface plasmons; Diffraction gratings; Biological sensing and sensors
n3:klicoveSlovo
n11:Diffraction%20gratings n11:Surface%20plasmons n11:Biological%20sensing%20and%20sensors
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[99392298E265]
n3:nazevZdroje
Optics Express
n3:obor
n4:BH
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:projekt
n18:GBP205%2F12%2FG118
n3:rokUplatneniVysledku
n5:2013
n3:svazekPeriodika
21
n3:tvurceVysledku
Dostálek, J. Homola, Jiří Knoll, W. Vala, Milan Toma, K. Adam, Pavel
n3:wos
000318151600088
s:issn
1094-4087
s:numberOfPages
12
n16:doi
10.1364/OE.21.010121