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Statements

Subject Item
n2:RIV%2F67985882%3A_____%2F11%3A00358220%21RIV11-MSM-67985882
rdf:type
n9:Vysledek skos:Concept
dcterms:description
This paper reports an approach to detection of single nucleotide polymorphism based on special amplification assay and surface plasmon resonance (SPR) biosensor technology. In this assay, a part of the target DNA is recognized by a probe coupled with streptavidin-oligonucleotide (SON) complexes ex-situ. The SON-target complex is then detected with the SPR sensor with high specificity and sensitivity. The assay is applied to detection of short analogue of TP53 at levels down to 40 pM. This paper reports an approach to detection of single nucleotide polymorphism based on special amplification assay and surface plasmon resonance (SPR) biosensor technology. In this assay, a part of the target DNA is recognized by a probe coupled with streptavidin-oligonucleotide (SON) complexes ex-situ. The SON-target complex is then detected with the SPR sensor with high specificity and sensitivity. The assay is applied to detection of short analogue of TP53 at levels down to 40 pM.
dcterms:title
Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53 Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53
skos:prefLabel
Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53 Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53
skos:notation
RIV/67985882:_____/11:00358220!RIV11-MSM-67985882
n9:predkladatel
n16:ico%3A67985882
n3:aktivita
n13:P n13:Z
n3:aktivity
P(GA202/09/0193), P(KAN200670701), P(OC09058), Z(AV0Z20670512)
n3:cisloPeriodika
7
n3:dodaniDat
n14:2011
n3:domaciTvurceVysledku
n4:2036444 n4:9632689 n4:4933672
n3:druhVysledku
n15:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
232602
n3:idVysledku
RIV/67985882:_____/11:00358220
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Surface Plasmon Resonance; point mutation; amplification
n3:klicoveSlovo
n8:point%20mutation n8:Surface%20Plasmon%20Resonance n8:amplification
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[15FAC76C63E3]
n3:nazevZdroje
Analytical and Bioanalytical Chemistry
n3:obor
n12:JB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n6:GA202%2F09%2F0193 n6:OC09058 n6:KAN200670701
n3:rokUplatneniVysledku
n14:2011
n3:svazekPeriodika
399
n3:tvurceVysledku
Homola, Jiří Šípová, Hana Špringer, Tomáš
n3:wos
000287141700006
n3:zamer
n7:AV0Z20670512
s:issn
1618-2642
s:numberOfPages
8