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Statements

Subject Item
n2:RIV%2F67985858%3A_____%2F15%3A00438314%21RIV15-TA0-67985858
rdf:type
n10:Vysledek skos:Concept
dcterms:description
A biosensor for putrescine containing a sensing layer with an optical oxygen probe based on ruthenium complex and the enzyme diamine oxidase from pea is described. The diamine oxidase was pre-immobilised on broken micro-beads modified with a ferrofluid. The pre-immobilised enzyme and ruthenium complex were both incorporated into the UV-cured inorganic–organic hybrid polymer ORMOCER and deposited on a lens to form a sensitive layer of 210 μm in thickness. The sensitivity to the putrescine concentration determined under air saturation was between 3.50 μs L mmol-1 and 4.50 μs L mmol-1 in a hundred experiments conducted intermittently over a one year period. With the oxygen concentration increasing from 10 % to 100 % of DO (dissolved oxygen), the biosensor sensitivity decreased from 6.87 μs L mmol-1 to 0.70 μs L mmol-1 and its dynamic range increased from 0.10 mmol L-1 to 1.75 mmol L-1. To estimate the behaviour of the putrescine sensor in parametric space, a mathematical model of the reaction–transport processes inside the sensing layer was developed. The model revealed the qualitative relations between the sensor analytical features, the characteristics of the sensitive layer and concentrations of substrates. The results of the mathematical modelling may serve as guidelines in the design of optodes for specific applications. A biosensor for putrescine containing a sensing layer with an optical oxygen probe based on ruthenium complex and the enzyme diamine oxidase from pea is described. The diamine oxidase was pre-immobilised on broken micro-beads modified with a ferrofluid. The pre-immobilised enzyme and ruthenium complex were both incorporated into the UV-cured inorganic–organic hybrid polymer ORMOCER and deposited on a lens to form a sensitive layer of 210 μm in thickness. The sensitivity to the putrescine concentration determined under air saturation was between 3.50 μs L mmol-1 and 4.50 μs L mmol-1 in a hundred experiments conducted intermittently over a one year period. With the oxygen concentration increasing from 10 % to 100 % of DO (dissolved oxygen), the biosensor sensitivity decreased from 6.87 μs L mmol-1 to 0.70 μs L mmol-1 and its dynamic range increased from 0.10 mmol L-1 to 1.75 mmol L-1. To estimate the behaviour of the putrescine sensor in parametric space, a mathematical model of the reaction–transport processes inside the sensing layer was developed. The model revealed the qualitative relations between the sensor analytical features, the characteristics of the sensitive layer and concentrations of substrates. The results of the mathematical modelling may serve as guidelines in the design of optodes for specific applications.
dcterms:title
Enzymatic Sensor of Putrescine with Optical Oxygen Transducer - Mathematical Model of Responses of Sensitive Layer. Enzymatic Sensor of Putrescine with Optical Oxygen Transducer - Mathematical Model of Responses of Sensitive Layer.
skos:prefLabel
Enzymatic Sensor of Putrescine with Optical Oxygen Transducer - Mathematical Model of Responses of Sensitive Layer. Enzymatic Sensor of Putrescine with Optical Oxygen Transducer - Mathematical Model of Responses of Sensitive Layer.
skos:notation
RIV/67985858:_____/15:00438314!RIV15-TA0-67985858
n3:aktivita
n17:P n17:I
n3:aktivity
I, P(TA03010548)
n3:cisloPeriodika
1
n3:dodaniDat
n9:2015
n3:domaciTvurceVysledku
n8:8231516 n8:4052501 n8:4739922 n8:3329194
n3:druhVysledku
n15:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
130
n3:idVysledku
RIV/67985858:_____/15:00438314
n3:jazykVysledku
n18:eng
n3:klicovaSlova
enzymatic sensor; putrescine; optical oxygen transducer
n3:klicoveSlovo
n4:putrescine n4:optical%20oxygen%20transducer n4:enzymatic%20sensor
n3:kodStatuVydavatele
SK - Slovenská republika
n3:kontrolniKodProRIV
[26DA9346DFDA]
n3:nazevZdroje
Chemical Papers
n3:obor
n12:CE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:projekt
n16:TA03010548
n3:rokUplatneniVysledku
n9:2015
n3:svazekPeriodika
69
n3:tvurceVysledku
Kuncová, Gabriela Přibyl, M. Koštejn, Martin Maixnerová, Lucie Šebela, M. Horvitz, Alexandar
n3:wos
000350130100014
s:issn
0366-6352
s:numberOfPages
9
n14:doi
10.1515/chempap-2015-0041