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Statements

Subject Item
n2:RIV%2F67985823%3A_____%2F13%3A00423804%21RIV14-AV0-67985823
rdf:type
n3:Vysledek skos:Concept
dcterms:description
The changes of photosynthetic performance caused by increasing CO2 concentration are refl ected at diff erent hierarchical levels of photosynthetic apparatus from the effi ciency of individual photosynthetic processes to changes in leaf structure. Th e aim of this study was to elucidate the relationships between the ultrastructure and function of photosynthetic apparatus under elevated CO2 concentration. We studied two common tree species – the European beech and the Norway spruce – grown under ambient (AC) and elevated CO2 concentrations (EC). Photosynthetic parameters were obtained using the gas-exchange system LI-6400 (LI-COR, USA). Th e characteristics of chlorophyll a fl uorescence were obtained in vivo with FluorPen FP100max (PSI, CZE) and in vitro polarographically in a suspension of isolated chloroplasts. Non-structural soluble saccharides and starch accumulation was quantifi ed using HPLC. Th e chloroplast ultrastructure was quantifi ed by stereological methods (Ellipse 2.08, SK) on the images acquired on a JEOL JEM-1011 (JEOL, JPN). Th e light-saturated CO2 assimilation rate was stimulated by EC in both species. Th e assimilation capacity of EC plants slightly decreased and was accompanied by a slight decrease in the rate of electron transport and the rate of Rubisco carboxylation. Th e growth in EC induced higher energy dissipation by light-harvesting antennae, diminished PSII activity, resulted in greater PSI capacity and in higher accumulation of starch and soluble sugars in the leaves of both species. The changes of photosynthetic performance caused by increasing CO2 concentration are refl ected at diff erent hierarchical levels of photosynthetic apparatus from the effi ciency of individual photosynthetic processes to changes in leaf structure. Th e aim of this study was to elucidate the relationships between the ultrastructure and function of photosynthetic apparatus under elevated CO2 concentration. We studied two common tree species – the European beech and the Norway spruce – grown under ambient (AC) and elevated CO2 concentrations (EC). Photosynthetic parameters were obtained using the gas-exchange system LI-6400 (LI-COR, USA). Th e characteristics of chlorophyll a fl uorescence were obtained in vivo with FluorPen FP100max (PSI, CZE) and in vitro polarographically in a suspension of isolated chloroplasts. Non-structural soluble saccharides and starch accumulation was quantifi ed using HPLC. Th e chloroplast ultrastructure was quantifi ed by stereological methods (Ellipse 2.08, SK) on the images acquired on a JEOL JEM-1011 (JEOL, JPN). Th e light-saturated CO2 assimilation rate was stimulated by EC in both species. Th e assimilation capacity of EC plants slightly decreased and was accompanied by a slight decrease in the rate of electron transport and the rate of Rubisco carboxylation. Th e growth in EC induced higher energy dissipation by light-harvesting antennae, diminished PSII activity, resulted in greater PSI capacity and in higher accumulation of starch and soluble sugars in the leaves of both species.
dcterms:title
Changes in the photosynthetic apparatus of european beech and Norway spruce under long-term exposure to elevated CO2 Changes in the photosynthetic apparatus of european beech and Norway spruce under long-term exposure to elevated CO2
skos:prefLabel
Changes in the photosynthetic apparatus of european beech and Norway spruce under long-term exposure to elevated CO2 Changes in the photosynthetic apparatus of european beech and Norway spruce under long-term exposure to elevated CO2
skos:notation
RIV/67985823:_____/13:00423804!RIV14-AV0-67985823
n3:predkladatel
n13:ico%3A67985823
n4:aktivita
n19:I
n4:aktivity
I
n4:dodaniDat
n12:2014
n4:domaciTvurceVysledku
n7:9785353
n4:druhVysledku
n16:D
n4:duvernostUdaju
n8:S
n4:entitaPredkladatele
n20:predkladatel
n4:idSjednocenehoVysledku
64945
n4:idVysledku
RIV/67985823:_____/13:00423804
n4:jazykVysledku
n18:eng
n4:klicovaSlova
photosynthetic apparatus; european beech; Norway spruce; elevated CO2
n4:klicoveSlovo
n6:european%20beech n6:elevated%20CO2 n6:photosynthetic%20apparatus n6:Norway%20spruce
n4:kontrolniKodProRIV
[A1D25DA6C0E4]
n4:mistoKonaniAkce
Brno
n4:mistoVydani
Brno
n4:nazevZdroje
Global Change and Resilience: From Impacts to Responses : Proceedings of the 3rd annual Global Change and Resilience Conference
n4:obor
n17:ED
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
11
n4:rokUplatneniVysledku
n12:2013
n4:tvurceVysledku
Šprtová, Mirka Lhotáková, Z. Albrechtová, J. Holá, D. Mašková, P. Lipavská, H. Kubásek, Jiří Kočová, M. Urban, Otmar Radochová, Barbora Holišová, Petra
n4:typAkce
n5:EUR
n4:zahajeniAkce
2013-05-22+02:00
s:numberOfPages
4
n14:hasPublisher
Global change research centre, Academy of Sciences of the Czech Republic, v. v. i
n11:isbn
978-80-904351-8-6