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Statements

Subject Item
n2:RIV%2F67985823%3A_____%2F11%3A00369489%21RIV12-AV0-67985823
rdf:type
n10:Vysledek skos:Concept
dcterms:description
In images acquired by confocal laser scanning microscopy (CLSM), regions corresponding to the same concentration of fluorophores in the specimen should be mapped to the same grayscale levels. However, in practice, due to multiple distortion effects, CLSM images of even homogeneous specimen regions suffer from irregular brightness variations, e.g., darkening of image edges and lightening of the center. We present a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain In images acquired by confocal laser scanning microscopy (CLSM), regions corresponding to the same concentration of fluorophores in the specimen should be mapped to the same grayscale levels. However, in practice, due to multiple distortion effects, CLSM images of even homogeneous specimen regions suffer from irregular brightness variations, e.g., darkening of image edges and lightening of the center. We present a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain
dcterms:title
Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy
skos:prefLabel
Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy
skos:notation
RIV/67985823:_____/11:00369489!RIV12-AV0-67985823
n10:predkladatel
n16:ico%3A67985823
n5:aktivita
n11:Z n11:P
n5:aktivity
P(GA102/08/0691), P(GA304/09/0733), P(LC06063), P(ME09010), Z(AV0Z50110509), Z(MSM6840770012)
n5:cisloPeriodika
9
n5:dodaniDat
n7:2012
n5:domaciTvurceVysledku
n12:1487221 n12:6691587 n12:3658023
n5:druhVysledku
n13:J
n5:duvernostUdaju
n19:S
n5:entitaPredkladatele
n8:predkladatel
n5:idSjednocenehoVysledku
191331
n5:idVysledku
RIV/67985823:_____/11:00369489
n5:jazykVysledku
n18:eng
n5:klicovaSlova
confocal laser scanning microscopy; image enhancement; morphology filters
n5:klicoveSlovo
n14:confocal%20laser%20scanning%20microscopy n14:morphology%20filters n14:image%20enhancement
n5:kodStatuVydavatele
US - Spojené státy americké
n5:kontrolniKodProRIV
[EE008501A8A4]
n5:nazevZdroje
Microscopy Research Technique
n5:obor
n20:JC
n5:pocetDomacichTvurcuVysledku
3
n5:pocetTvurcuVysledku
3
n5:projekt
n15:GA102%2F08%2F0691 n15:LC06063 n15:ME09010 n15:GA304%2F09%2F0733
n5:rokUplatneniVysledku
n7:2011
n5:svazekPeriodika
74
n5:tvurceVysledku
Michálek, Jan Kubínová, Lucie Čapek, Martin
n5:wos
000294401200007
n5:zamer
n6:MSM6840770012 n6:AV0Z50110509
s:issn
1059-910X
s:numberOfPages
8
n17:doi
10.1002/jemt.20965