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Statements

Subject Item
n2:RIV%2F67985823%3A_____%2F10%3A00345607%21RIV11-MSM-67985823
rdf:type
n6:Vysledek skos:Concept
dcterms:description
We constructed a new pHluorin plasmid with the dominant selection marker (KanMX). This plasmid allows pH measurements in cells without auxotrophic mutations and/or grown in chemically indefinite media. We also utilised pHluorin in a new method of measuring the buffering capacity of yeast cell cytosol in vivo We constructed a new pHluorin plasmid with the dominant selection marker (KanMX). This plasmid allows pH measurements in cells without auxotrophic mutations and/or grown in chemically indefinite media. We also utilised pHluorin in a new method of measuring the buffering capacity of yeast cell cytosol in vivo
dcterms:title
New applications of pHluorin - measuring intracellular pH of prototrophic yeasts and determining changes in the buffering capacity of strains with affected potassium homeostasis New applications of pHluorin - measuring intracellular pH of prototrophic yeasts and determining changes in the buffering capacity of strains with affected potassium homeostasis
skos:prefLabel
New applications of pHluorin - measuring intracellular pH of prototrophic yeasts and determining changes in the buffering capacity of strains with affected potassium homeostasis New applications of pHluorin - measuring intracellular pH of prototrophic yeasts and determining changes in the buffering capacity of strains with affected potassium homeostasis
skos:notation
RIV/67985823:_____/10:00345607!RIV11-MSM-67985823
n3:aktivita
n4:P n4:Z
n3:aktivity
P(KJB500110701), P(LC531), Z(AV0Z50110509), Z(MSM0021620835)
n3:cisloPeriodika
6
n3:dodaniDat
n16:2011
n3:domaciTvurceVysledku
n11:1112368 n11:9978194
n3:druhVysledku
n18:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
274599
n3:idVysledku
RIV/67985823:_____/10:00345607
n3:jazykVysledku
n17:eng
n3:klicovaSlova
pH maintenance; Saccharomyces cerevisiae; fluorescence measurements
n3:klicoveSlovo
n15:pH%20maintenance n15:Saccharomyces%20cerevisiae n15:fluorescence%20measurements
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[E28B2AEB62ED]
n3:nazevZdroje
Yeast
n3:obor
n12:ED
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
5
n3:projekt
n13:KJB500110701 n13:LC531
n3:rokUplatneniVysledku
n16:2010
n3:svazekPeriodika
27
n3:tvurceVysledku
Sychrová, Hana Hošková, B. Urbánková, E. Chaloupka, R. Marešová, Lydie
n3:wos
279060200002
n3:zamer
n5:MSM0021620835 n5:AV0Z50110509
s:issn
0749-503X
s:numberOfPages
9