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Statements

Subject Item
n2:RIV%2F67985823%3A_____%2F10%3A00341849%21RIV11-GA0-67985823
rdf:type
n12:Vysledek skos:Concept
dcterms:description
We compared two methods of evaluation of capillary supply of individual skeletal muscle fibers, the traditional one using 2D images of thin transverse sections by the number of capillary profiles around a fiber (CAF) and the 3D approach where the mean length of capillaries around individual muscle fibers per fiber length (Lcap/Lfib) is measured from 3D images acquired by confocal microscopy. In models we showed that Lcap/Lfib was sensitive to different arrangements of capillaries, while CAF underestimated capillarization since it could not detect the increased length of capillary bed. In true muscle samples, we detected statistically significant differences in the capillary supply of control and denervated rat soleus muscles by both 2D and 3D methods. Lcap/Lfib was larger than CAF in control muscles reflecting their more complicated capillary bed We compared two methods of evaluation of capillary supply of individual skeletal muscle fibers, the traditional one using 2D images of thin transverse sections by the number of capillary profiles around a fiber (CAF) and the 3D approach where the mean length of capillaries around individual muscle fibers per fiber length (Lcap/Lfib) is measured from 3D images acquired by confocal microscopy. In models we showed that Lcap/Lfib was sensitive to different arrangements of capillaries, while CAF underestimated capillarization since it could not detect the increased length of capillary bed. In true muscle samples, we detected statistically significant differences in the capillary supply of control and denervated rat soleus muscles by both 2D and 3D methods. Lcap/Lfib was larger than CAF in control muscles reflecting their more complicated capillary bed
dcterms:title
The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod
skos:prefLabel
The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod
skos:notation
RIV/67985823:_____/10:00341849!RIV11-GA0-67985823
n3:aktivita
n13:Z n13:P
n3:aktivity
P(GD204/09/H084), P(LC06063), P(MEB090910), Z(AV0Z50110509)
n3:cisloPeriodika
1
n3:dodaniDat
n15:2011
n3:domaciTvurceVysledku
n7:4096193 n7:1212613 n7:6691587
n3:druhVysledku
n17:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
257544
n3:idVysledku
RIV/67985823:_____/10:00341849
n3:jazykVysledku
n16:eng
n3:klicovaSlova
confocal microscopy; capillaries; length measurement
n3:klicoveSlovo
n10:length%20measurement n10:capillaries n10:confocal%20microscopy
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[B4FC84DA7392]
n3:nazevZdroje
Microvascular Research
n3:obor
n14:EA
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:projekt
n9:MEB090910 n9:GD204%2F09%2FH084 n9:LC06063
n3:rokUplatneniVysledku
n15:2010
n3:svazekPeriodika
79
n3:tvurceVysledku
Čebašek, V. Vyhnal, Aleš Eržen, I. Kubínová, Lucie Ribarič, S. Janáček, Jiří
n3:wos
274055200006
n3:zamer
n8:AV0Z50110509
s:issn
0026-2862
s:numberOfPages
7