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Statements

Subject Item
n2:RIV%2F67985823%3A_____%2F08%3A00315027%21RIV09-AV0-67985823
rdf:type
skos:Concept n14:Vysledek
dcterms:description
PCR was performed on the cistron of rDNA in the two groups of infective larvae Trichostrongylus colubriformis —the population with and without ability to undergo arrested development. General primers designed by Caenorhabditis elegans rDNA were used for the amplification of the rDNA cistron between genes 18S and 28S. Amplified fragments were digested by using a series of restriction endonucleases. Hinc II restriction profiles unique for each T. colubriformis populations were observed, and therefore enzyme Hinc II appears to be useful for the determination of populations with and without the ability to undergo arrested development PCR was performed on the cistron of rDNA in the two groups of infective larvae Trichostrongylus colubriformis —the population with and without ability to undergo arrested development. General primers designed by Caenorhabditis elegans rDNA were used for the amplification of the rDNA cistron between genes 18S and 28S. Amplified fragments were digested by using a series of restriction endonucleases. Hinc II restriction profiles unique for each T. colubriformis populations were observed, and therefore enzyme Hinc II appears to be useful for the determination of populations with and without the ability to undergo arrested development PCR reakce byla provedena na cistronu rDNA u dvou skupin infekčních larev Trichostrongylus colubriformis - populace se schopností pozastavit vývoj a populace bez této schopnosti. Primery navržené pro rDNA Caenorhabditis elegans byly použity pro amplifikaci cistronu rDNA mezi geny 18S a 28S. Amplifikované úseky byly natráveny sérií restrikčních endonukleáz. U obou populací T. colubriformis byly po natrávení enzymem Hinc II sledovány unikátní profily. HincII by mohl být vhodným enzymem pro determinaci populací se schopností podstoupit hypobiózu a bez této schopnosti
dcterms:title
Trichostrongylus colubriformis rDNA polymorphism associated with arrested development Trichostrongylus colubriformis rDNA polymorphism associated with arrested development Polymorfizmus rDNA Trichostrongylus colubriformis spojený s hypobiózou
skos:prefLabel
Trichostrongylus colubriformis rDNA polymorphism associated with arrested development Polymorfizmus rDNA Trichostrongylus colubriformis spojený s hypobiózou Trichostrongylus colubriformis rDNA polymorphism associated with arrested development
skos:notation
RIV/67985823:_____/08:00315027!RIV09-AV0-67985823
n4:aktivita
n12:Z
n4:aktivity
Z(AV0Z50110509)
n4:cisloPeriodika
2
n4:dodaniDat
n11:2009
n4:domaciTvurceVysledku
n17:3935035
n4:druhVysledku
n16:J
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n7:predkladatel
n4:idSjednocenehoVysledku
400708
n4:idVysledku
RIV/67985823:_____/08:00315027
n4:jazykVysledku
n6:eng
n4:klicovaSlova
arrested development; polymorphism; rDNA
n4:klicoveSlovo
n8:arrested%20development n8:polymorphism n8:rDNA
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[6D1E159AF575]
n4:nazevZdroje
Parasitology Research
n4:obor
n9:EB
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
6
n4:rokUplatneniVysledku
n11:2008
n4:svazekPeriodika
103
n4:tvurceVysledku
Borovský, M. Jankovská, I. Vadlejch, J. Lytvynets, Andriy Langrová, I. Zouhar, M.
n4:wos
256091600024
n4:zamer
n13:AV0Z50110509
s:issn
0932-0113
s:numberOfPages
3