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Statements

Subject Item
n2:RIV%2F67985823%3A_____%2F07%3A00091032%21RIV08-AV0-67985823
rdf:type
n8:Vysledek skos:Concept
dcterms:description
A set of tools for the genetic manipulation of the osmotolerant yeast Zygosaccharomyces rouxii was developed. Auxotrophic mutants (ura3 leu2, ura3 ade2, ura3 leu2 ade2) derived from the CBS 732 type strain were prepared. Centromeric and episomal Z. rouxii/E. coli shuttle plasmids with different marker genes (ScURA3, ZrLEU2, ZrADE2) and with multiple cloning sites were constructed, together with a plasmid enabling GFP-tagging. A system for repeatable targeted gene deletion in Z. rouxii was established A set of tools for the genetic manipulation of the osmotolerant yeast Zygosaccharomyces rouxii was developed. Auxotrophic mutants (ura3 leu2, ura3 ade2, ura3 leu2 ade2) derived from the CBS 732 type strain were prepared. Centromeric and episomal Z. rouxii/E. coli shuttle plasmids with different marker genes (ScURA3, ZrLEU2, ZrADE2) and with multiple cloning sites were constructed, together with a plasmid enabling GFP-tagging. A system for repeatable targeted gene deletion in Z. rouxii was established Byl připraven soubor nástrojů genového inženýrství pro kvasinku Zygosaccharomyces rouxii. Byly zkonstruovány auxotrofní mutantní kmeny spolu s centromerovými a episomálními vektory a vyvinut system umožňující opakovatelné delece
dcterms:title
Tools for the genetic manipulation of Zygosaccharomyces rouxii Tools for the genetic manipulation of Zygosaccharomyces rouxii Nástroje genového inženýrství pro kvasinku Zygosaccharomyces rouxii
skos:prefLabel
Nástroje genového inženýrství pro kvasinku Zygosaccharomyces rouxii Tools for the genetic manipulation of Zygosaccharomyces rouxii Tools for the genetic manipulation of Zygosaccharomyces rouxii
skos:notation
RIV/67985823:_____/07:00091032!RIV08-AV0-67985823
n4:strany
1285;1294
n4:aktivita
n12:Z n12:P
n4:aktivity
P(GA204/05/0028), Z(AV0Z50110509)
n4:cisloPeriodika
8
n4:dodaniDat
n9:2008
n4:domaciTvurceVysledku
n10:7804199 n10:9978194
n4:druhVysledku
n14:J
n4:duvernostUdaju
n11:S
n4:entitaPredkladatele
n15:predkladatel
n4:idSjednocenehoVysledku
455322
n4:idVysledku
RIV/67985823:_____/07:00091032
n4:jazykVysledku
n18:eng
n4:klicovaSlova
Zygosaccharomyces rouxii; genetic tools; plasmids
n4:klicoveSlovo
n5:genetic%20tools n5:plasmids n5:Zygosaccharomyces%20rouxii
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[C2420E81FCCB]
n4:nazevZdroje
FEMS Yeast Research
n4:obor
n17:EB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
3
n4:projekt
n6:GA204%2F05%2F0028
n4:rokUplatneniVysledku
n9:2007
n4:svazekPeriodika
7
n4:tvurceVysledku
Přibylová, Lenka de Montigny, J. Sychrová, Hana
n4:zamer
n16:AV0Z50110509
s:issn
1567-1356
s:numberOfPages
10