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Statements

Subject Item
n2:RIV%2F67179843%3A_____%2F12%3A00388804%21RIV13-AV0-67179843
rdf:type
skos:Concept n13:Vysledek
dcterms:description
Drought stress is one of the most important factors that limit crop productivity worldwide. In order to obtain tomato plants with enhanced drought tolerance, we inserted the transcription factor gene ATHB-7 into the tomato genome. This gene was demonstrated earlier to be up-regulated during drought stress in Arabidopsis thaliana thus acting as a negative regulator of growth. We compared the performance of wild type and transgenic tomato line DTL-20, carrying ATHB-7 gene, under well-irrigated and water limited conditions. We found that transgenic plants had reduced stomatal density and stomatal pore size and exhibited an enhanced resistance to soil water deficit. We used the transgenic plants to investigate the potential of chlorophyll fluorescence to report drought tolerance in a simulated high-throughput screening procedure. Wild type and transgenic tomato plants were exposed to drought stress lasting 18 days. The stress was then terminated by rehydration after which recovery was studied for another 2 days. Plant growth, leaf water potential, and chlorophyll fluorescence were measured during the entire experimental period. We found that water potential in wild type and drought tolerant transgenic plants diverged around day 11 of induced drought stress. The chlorophyll fluorescence parameters: the non-photochemical quenching, effective quantum efficiency of PSII, and the maximum quantum yield of PSII photochemistry yielded a good contrast between wild type and transgenic plants from day 7, day 12, and day 14 of induced stress, respectively. We propose that chlorophyll fluorescence emission reports well on the level of water stress and, thus, can be used to identify elevated drought tolerance in high-throughput screens for selection of resistant genotypes. Drought stress is one of the most important factors that limit crop productivity worldwide. In order to obtain tomato plants with enhanced drought tolerance, we inserted the transcription factor gene ATHB-7 into the tomato genome. This gene was demonstrated earlier to be up-regulated during drought stress in Arabidopsis thaliana thus acting as a negative regulator of growth. We compared the performance of wild type and transgenic tomato line DTL-20, carrying ATHB-7 gene, under well-irrigated and water limited conditions. We found that transgenic plants had reduced stomatal density and stomatal pore size and exhibited an enhanced resistance to soil water deficit. We used the transgenic plants to investigate the potential of chlorophyll fluorescence to report drought tolerance in a simulated high-throughput screening procedure. Wild type and transgenic tomato plants were exposed to drought stress lasting 18 days. The stress was then terminated by rehydration after which recovery was studied for another 2 days. Plant growth, leaf water potential, and chlorophyll fluorescence were measured during the entire experimental period. We found that water potential in wild type and drought tolerant transgenic plants diverged around day 11 of induced drought stress. The chlorophyll fluorescence parameters: the non-photochemical quenching, effective quantum efficiency of PSII, and the maximum quantum yield of PSII photochemistry yielded a good contrast between wild type and transgenic plants from day 7, day 12, and day 14 of induced stress, respectively. We propose that chlorophyll fluorescence emission reports well on the level of water stress and, thus, can be used to identify elevated drought tolerance in high-throughput screens for selection of resistant genotypes.
dcterms:title
Engineered drought tolerance in tomato plants is reflected in chlorophyll fluorescence emission Engineered drought tolerance in tomato plants is reflected in chlorophyll fluorescence emission
skos:prefLabel
Engineered drought tolerance in tomato plants is reflected in chlorophyll fluorescence emission Engineered drought tolerance in tomato plants is reflected in chlorophyll fluorescence emission
skos:notation
RIV/67179843:_____/12:00388804!RIV13-AV0-67179843
n13:predkladatel
n16:ico%3A67179843
n3:aktivita
n19:P n19:I
n3:aktivity
I, P(2B06068), P(ED1.1.00/02.0073), P(OC08055)
n3:cisloPeriodika
SI
n3:dodaniDat
n9:2013
n3:domaciTvurceVysledku
n12:3910830 n12:3169189 n12:7311877
n3:druhVysledku
n17:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
134398
n3:idVysledku
RIV/67179843:_____/12:00388804
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Chlorophyll fluorescence; Drought; High-throughput screening; Solanum lycopersicum; Transcription factor; Transgenic plant
n3:klicoveSlovo
n4:Solanum%20lycopersicum n4:High-throughput%20screening n4:Drought n4:Transgenic%20plant n4:Chlorophyll%20fluorescence n4:Transcription%20factor
n3:kodStatuVydavatele
IE - Irsko
n3:kontrolniKodProRIV
[8364D04D9C46]
n3:nazevZdroje
Plant Science
n3:obor
n11:EH
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
9
n3:projekt
n10:ED1.1.00%2F02.0073 n10:OC08055 n10:2B06068
n3:rokUplatneniVysledku
n9:2012
n3:svazekPeriodika
182
n3:tvurceVysledku
Trtílek, M. Nedbal, Ladislav Mishra, Anamika Armentano, N. Cellini, F. Mishra, Kumud Petrozza, A. Iannacone, R. La Vecchia, G.
n3:wos
000298723200010
s:issn
0168-9452
s:numberOfPages
8
n18:doi
10.1016/j.plantsci.2011.03.022