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Statements

Subject Item
n2:RIV%2F67179843%3A_____%2F12%3A00379290%21RIV13-AV0-67179843
rdf:type
n12:Vysledek skos:Concept
dcterms:description
We studied Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) content and activity in juvenile tree species - broadleaved Fagus sylvatica L. and coniferous Picea abies (L.) Karsten exposed for three growing seasons to ambient (A = 385 mu mol mol(-1)) and elevated (E = 700 mu mol mol(-1)) CO2 concentrations. Rubisco content was determined by the SDS-PAGE method, Rubisco activity was assayed spectrophotometrically. The highest content of Rubisco enzyme in F. sylvatica was measured immediately after full leaf development followed by a gradual decrease throughout the growing season. By contrast, Rubisco content in P. abies increased markedly during the growing season. In both tree species, down-regulation of Rubisco content in trees cultivated under elevated CO2 concentration was observed. Rubisco activity was stimulated in F. sylvatica by E treatment but not in P. abies. Because no significant differences were found in Rubisco activation state between A and E, we assume that stimulation of Rubisco activity in E is not a consequence of higher carbamylation but could be caused by the release of inhibitors from active sites of Rubisco under elevated CO2. We studied Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) content and activity in juvenile tree species - broadleaved Fagus sylvatica L. and coniferous Picea abies (L.) Karsten exposed for three growing seasons to ambient (A = 385 mu mol mol(-1)) and elevated (E = 700 mu mol mol(-1)) CO2 concentrations. Rubisco content was determined by the SDS-PAGE method, Rubisco activity was assayed spectrophotometrically. The highest content of Rubisco enzyme in F. sylvatica was measured immediately after full leaf development followed by a gradual decrease throughout the growing season. By contrast, Rubisco content in P. abies increased markedly during the growing season. In both tree species, down-regulation of Rubisco content in trees cultivated under elevated CO2 concentration was observed. Rubisco activity was stimulated in F. sylvatica by E treatment but not in P. abies. Because no significant differences were found in Rubisco activation state between A and E, we assume that stimulation of Rubisco activity in E is not a consequence of higher carbamylation but could be caused by the release of inhibitors from active sites of Rubisco under elevated CO2.
dcterms:title
Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration
skos:prefLabel
Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration
skos:notation
RIV/67179843:_____/12:00379290!RIV13-AV0-67179843
n12:predkladatel
n13:ico%3A67179843
n3:aktivita
n4:I n4:Z n4:P
n3:aktivity
I, P(ED1.1.00/02.0073), P(IAA600870701), P(LM2010007), Z(AV0Z60870520)
n3:cisloPeriodika
9
n3:dodaniDat
n16:2013
n3:domaciTvurceVysledku
n17:3280551
n3:druhVysledku
n8:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
167113
n3:idVysledku
RIV/67179843:_____/12:00379290
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Rubisco content; Rubisco activity; seasonal changes; elevated CO2 concentrations; Fagus sylvatica; Picea abies
n3:klicoveSlovo
n7:Picea%20abies n7:elevated%20CO2%20concentrations n7:Fagus%20sylvatica n7:Rubisco%20activity n7:Rubisco%20content n7:seasonal%20changes
n3:kodStatuVydavatele
SK - Slovenská republika
n3:kontrolniKodProRIV
[B7629D7306C6]
n3:nazevZdroje
Chemical Papers
n3:obor
n10:EH
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
3
n3:projekt
n9:ED1.1.00%2F02.0073 n9:IAA600870701 n9:LM2010007
n3:rokUplatneniVysledku
n16:2012
n3:svazekPeriodika
66
n3:tvurceVysledku
Urban, Otmar Hrstka, M. Babák, L.
n3:wos
000305563300006
n3:zamer
n20:AV0Z60870520
s:issn
0366-6352
s:numberOfPages
6
n18:doi
10.2478/s11696-012-0188-5