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Statements

Subject Item
n2:RIV%2F67179843%3A_____%2F10%3A00355844%21RIV11-GA0-67179843
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Two genes in the genome of Aspergillus niger, aglA and aglB, have been assigned to encode for α-d-galactosidases variant A and B. However, analyses of primary and 3D structures based on structural models of these two enzymes revealed significant differences in their active centers suggesting important differences in their specificity for the hydrolyzed carbohydrates. To test this unexpected finding, a large screening of libraries from 42 strains of filamentous fungi succeeded in identifying an enzyme from A. niger CCIM K2 that exhibited both α-galactosidase andα-N-acetylgalactosaminidase activities, with the latter activity predominating. The enzyme protein was sequenced, and its amino acid sequence could be unequivocally assigned to the enzyme encoded the aglA gene. Enzyme activity measurements and substrate docking clearly demonstrated the preference of the identified enzyme for α-N-acetyl-d-galactosaminide over α-d-galactoside. Two genes in the genome of Aspergillus niger, aglA and aglB, have been assigned to encode for α-d-galactosidases variant A and B. However, analyses of primary and 3D structures based on structural models of these two enzymes revealed significant differences in their active centers suggesting important differences in their specificity for the hydrolyzed carbohydrates. To test this unexpected finding, a large screening of libraries from 42 strains of filamentous fungi succeeded in identifying an enzyme from A. niger CCIM K2 that exhibited both α-galactosidase andα-N-acetylgalactosaminidase activities, with the latter activity predominating. The enzyme protein was sequenced, and its amino acid sequence could be unequivocally assigned to the enzyme encoded the aglA gene. Enzyme activity measurements and substrate docking clearly demonstrated the preference of the identified enzyme for α-N-acetyl-d-galactosaminide over α-d-galactoside.
dcterms:title
The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase
skos:prefLabel
The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase
skos:notation
RIV/67179843:_____/10:00355844!RIV11-GA0-67179843
n3:aktivita
n10:S n10:P n10:I n10:Z
n3:aktivity
I, P(GA303/09/0477), P(GAP207/10/0321), P(GAP207/10/1040), P(GAP207/10/1934), P(GD305/09/H008), P(LC06010), S, Z(AV0Z10750506), Z(AV0Z50200510), Z(AV0Z60870520), Z(MSM0021620808), Z(MSM6007665808)
n3:cisloPeriodika
11
n3:dodaniDat
n13:2011
n3:domaciTvurceVysledku
n8:6326633 n8:1024868
n3:druhVysledku
n7:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
246010
n3:idVysledku
RIV/67179843:_____/10:00355844
n3:jazykVysledku
n17:eng
n3:klicovaSlova
α-N-acetylgalactosaminidase; α-galactosidase; Aspergillus niger; substrate binding; molecular modeling
n3:klicoveSlovo
n5:substrate%20binding n5:Aspergillus%20niger n5:molecular%20modeling n5:%CE%B1-N-acetylgalactosaminidase n5:%CE%B1-galactosidase
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[21881B53448F]
n3:nazevZdroje
Glycobiology
n3:obor
n18:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
10
n3:projekt
n4:GAP207%2F10%2F0321 n4:LC06010 n4:GAP207%2F10%2F1040 n4:GA303%2F09%2F0477 n4:GAP207%2F10%2F1934 n4:GD305%2F09%2FH008
n3:rokUplatneniVysledku
n13:2010
n3:svazekPeriodika
20
n3:tvurceVysledku
Bezouška, K. Křen, Vladimír Pompach, Petr Weignerová, L. Filipi, Tomáš Slámová, Kristýna Novák, Petr Ettrich, Rüdiger Mrázek, H. Kulik, Natallia
n3:wos
000282750300009
n3:zamer
n9:MSM6007665808 n9:AV0Z60870520 n9:AV0Z10750506 n9:MSM0021620808 n9:AV0Z50200510
s:issn
0959-6658
s:numberOfPages
10