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Statements

Subject Item
n2:RIV%2F65269705%3A_____%2F10%3A%230000946%21RIV11-MZ0-65269705
rdf:type
n13:Vysledek skos:Concept
dcterms:description
Sequence-specific DNA binding is the key function through which tumor suppressor p53 exerts transactivation of the downstream target genes, often being impaired in cancer cells by mutations in the TP53 gene. Functional protein microarray technology enables a high-throughput parallel analysis of protein properties within one experiment under the same conditions. Using an array approach, we analyzed the DNA binding activity of wild type p53 protein and of 49 variants. Our results show significant differences in the binding properties between the p53 mutants. The C-terminal mutant R337C displayed the highest DNA binding activity on the array. However, the same mutant showed only a partial activation in the reporter gene assay and almost no activation of downstream target genes after transfection of expression vector into cells lacking endogenous p53 Sequence-specific DNA binding is the key function through which tumor suppressor p53 exerts transactivation of the downstream target genes, often being impaired in cancer cells by mutations in the TP53 gene. Functional protein microarray technology enables a high-throughput parallel analysis of protein properties within one experiment under the same conditions. Using an array approach, we analyzed the DNA binding activity of wild type p53 protein and of 49 variants. Our results show significant differences in the binding properties between the p53 mutants. The C-terminal mutant R337C displayed the highest DNA binding activity on the array. However, the same mutant showed only a partial activation in the reporter gene assay and almost no activation of downstream target genes after transfection of expression vector into cells lacking endogenous p53
dcterms:title
Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation
skos:prefLabel
Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation
skos:notation
RIV/65269705:_____/10:#0000946!RIV11-MZ0-65269705
n4:aktivita
n5:P n5:Z
n4:aktivity
P(NS10439), P(NS9858), Z(MSM0021622412), Z(MSM0021622430)
n4:cisloPeriodika
2/3
n4:dodaniDat
n11:2011
n4:domaciTvurceVysledku
n12:2093995 n12:7997825 n12:1957864 n12:3169561 n12:4629604 n12:4376285
n4:druhVysledku
n18:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
246487
n4:idVysledku
RIV/65269705:_____/10:#0000946
n4:jazykVysledku
n9:eng
n4:klicovaSlova
R337C
n4:klicoveSlovo
n10:R337C
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[41BAAEABE7CD]
n4:nazevZdroje
Biological Chemistry
n4:obor
n16:FD
n4:pocetDomacichTvurcuVysledku
6
n4:pocetTvurcuVysledku
7
n4:projekt
n15:NS9858 n15:NS10439
n4:rokUplatneniVysledku
n11:2010
n4:svazekPeriodika
391
n4:tvurceVysledku
Mayer, Jiří Kabathová, Jitka Malčíková, Jitka Tichý, Boris Trbušek, Martin Pospíšilová, Šárka
n4:wos
000275194300009
n4:zamer
n6:MSM0021622412 n6:MSM0021622430
s:issn
1431-6730
s:numberOfPages
9