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Statements

Subject Item
n2:RIV%2F63221667%3A_____%2F09%3A%230000026%21RIV10-MZ0-63221667
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Cyclooxygenases are key enzymes in the arachidonic acid metabolism. Their unstable intermediate, prostaglandin H(2), is further metabolized to bioactive lipids by various downstream enzymes. In this study, utilizing short hairpin RNAs, we prepared a cell line of human cervix carcinoma with stable down-regulated cyclooxygenase-1 (COX-1) to assess the impact of COX-1 reduction on the downstream enzymes. We found a significant microsomal prostaglandin E synthase-1 (mPGES-1) suppression. In addition, mRNA expression of multidrug resistance protein 4 (MRP4, ABCC4), supposed to take part in antiviral and anticancer drug transport from cells, was up-regulated after COX-1 down-regulation. Our findings indicate that mPGES-1, believed to be coexpressed preferentially with cyclooxygenase-2, may be coupled to COX-1. ABCC4 up-regulation further supports the assumption of its involvement in prostanoid transport. Cyclooxygenases are key enzymes in the arachidonic acid metabolism. Their unstable intermediate, prostaglandin H(2), is further metabolized to bioactive lipids by various downstream enzymes. In this study, utilizing short hairpin RNAs, we prepared a cell line of human cervix carcinoma with stable down-regulated cyclooxygenase-1 (COX-1) to assess the impact of COX-1 reduction on the downstream enzymes. We found a significant microsomal prostaglandin E synthase-1 (mPGES-1) suppression. In addition, mRNA expression of multidrug resistance protein 4 (MRP4, ABCC4), supposed to take part in antiviral and anticancer drug transport from cells, was up-regulated after COX-1 down-regulation. Our findings indicate that mPGES-1, believed to be coexpressed preferentially with cyclooxygenase-2, may be coupled to COX-1. ABCC4 up-regulation further supports the assumption of its involvement in prostanoid transport.
dcterms:title
COX-1 is coupled with mPGES-1 and ABCC4 in human cervix cancer cells COX-1 is coupled with mPGES-1 and ABCC4 in human cervix cancer cells
skos:prefLabel
COX-1 is coupled with mPGES-1 and ABCC4 in human cervix cancer cells COX-1 is coupled with mPGES-1 and ABCC4 in human cervix cancer cells
skos:notation
RIV/63221667:_____/09:#0000026!RIV10-MZ0-63221667
n3:aktivita
n12:S n12:P n12:Z
n3:aktivity
P(NR8760), S, Z(MSM0021620820)
n3:cisloPeriodika
1-2
n3:dodaniDat
n5:2010
n3:domaciTvurceVysledku
n4:9812318 n4:8042470 n4:7860838 n4:9948066 n4:8624771 n4:3756378 n4:2132737
n3:druhVysledku
n13:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
308469
n3:idVysledku
RIV/63221667:_____/09:#0000026
n3:jazykVysledku
n9:eng
n3:klicovaSlova
Cyclooxygenase; Prostaglandin E synthase; mPGES-1; Multidrug resistance protein; ABCC4; shRNA
n3:klicoveSlovo
n11:shRNA n11:Multidrug%20resistance%20protein n11:mPGES-1 n11:Prostaglandin%20E%20synthase n11:ABCC4 n11:Cyclooxygenase
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[BFD3F5D7A9C0]
n3:nazevZdroje
Mol Cell Biochem
n3:obor
n16:EB
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
7
n3:projekt
n10:NR8760
n3:rokUplatneniVysledku
n5:2009
n3:svazekPeriodika
330
n3:tvurceVysledku
Radilová, Hana Kunc, Filip Holasová, Šárka Šafářová, Martina Libra, Antonín Bunček, Martin Víšková, Alena
n3:zamer
n18:MSM0021620820
s:issn
0300-8177
s:numberOfPages
10