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Statements

Subject Item
n2:RIV%2F62690094%3A18470%2F13%3A50001708%21RIV14-MSM-18470___
rdf:type
skos:Concept n17:Vysledek
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0278691513006480
dcterms:description
Ochratoxin A (OTA) is one of the most naturally occurring fungal toxins in food. It has been detected in high concentrations in serum samples of nephropathic patients and can be applied as one of the markers of potential risk of this disease. Also, OTA can cause adverse effects on human health such as genotoxicity and is anticipated to be a potential human carcinogen. In this study, enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) were applied in analysis of 115 blood serum samples of women in the child rearing age from the Czech Republic and both methods were compared. The OTA was presented in a broad range of concentrations from 0,037 to 1,130 ug/L. The outcome of ELISA and HPLC measurements were well correlated (r = 0.907). However, it was observed that ELISA, tend to result in underestimating the OTA level at the low serum concentrations. Both methods had the same limits of quantification of 0.050ug/L under standard operation conditions. When OTA concentration in a sample was too low, the sample was redissolved in only 300 uL of methanol and the detection limit for HPLC was lowered to 0.030 ug OTA/L. Ochratoxin A (OTA) is one of the most naturally occurring fungal toxins in food. It has been detected in high concentrations in serum samples of nephropathic patients and can be applied as one of the markers of potential risk of this disease. Also, OTA can cause adverse effects on human health such as genotoxicity and is anticipated to be a potential human carcinogen. In this study, enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) were applied in analysis of 115 blood serum samples of women in the child rearing age from the Czech Republic and both methods were compared. The OTA was presented in a broad range of concentrations from 0,037 to 1,130 ug/L. The outcome of ELISA and HPLC measurements were well correlated (r = 0.907). However, it was observed that ELISA, tend to result in underestimating the OTA level at the low serum concentrations. Both methods had the same limits of quantification of 0.050ug/L under standard operation conditions. When OTA concentration in a sample was too low, the sample was redissolved in only 300 uL of methanol and the detection limit for HPLC was lowered to 0.030 ug OTA/L.
dcterms:title
A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic
skos:prefLabel
A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic
skos:notation
RIV/62690094:18470/13:50001708!RIV14-MSM-18470___
n17:predkladatel
n18:orjk%3A18470
n3:aktivita
n9:S
n3:aktivity
S
n3:cisloPeriodika
12.09.2013
n3:dodaniDat
n15:2014
n3:domaciTvurceVysledku
n5:5323398 n5:3289133 n5:8812853
n3:druhVysledku
n6:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
58501
n3:idVysledku
RIV/62690094:18470/13:50001708
n3:jazykVysledku
n7:eng
n3:klicovaSlova
Serum; HPLC; ELISA; Ochratoxin A
n3:klicoveSlovo
n10:ELISA n10:Ochratoxin%20A n10:Serum n10:HPLC
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[3EF9159920D0]
n3:nazevZdroje
Food and chemical toxicology
n3:obor
n11:DN
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:rokUplatneniVysledku
n15:2013
n3:svazekPeriodika
62
n3:tvurceVysledku
Malíř, František Roubal, Tomáš Dohnal, Vlastimil Dvořák, Vladimír Ostrý, Vladimír
n3:wos
000329960400056
s:issn
0278-6915
s:numberOfPages
5
n14:doi
10.1016/j.fct.2013.09.010
n19:organizacniJednotka
18470