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Statements

Subject Item
n2:RIV%2F62157124%3A16810%2F14%3A43872648%21RIV15-MSM-16810___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Rural areas of Romania, particularly the localities covering Danube Delta, are still not sufficiently explored in terms of epidemiological aspects, despite the large density of domestic animals living in close contact with people and natural environment of the Danube Delta Biosphere Reserve. Between 2010 and 2012, a survey on equine piroplasmids species was performed in this area, using a total of 178 horses, 15 donkeys and 177 dogs from 18 localities inside and outside the Danube Delta. None of the sampled hosts showed any clinical symptoms typical for equine piroplasmoses. A 25.4% overall prevalence for both equine species of piroplasmids was detected by PCR. Detection by %22catch-all%22 primers followed by multiplex PCR revealed 20.3% prevalence of T. equi, beside 2.2% of B. caballi and 3.0% of mixed infections for all examined animals. Based on sequencing of 67 PCR amplicons of 18S rRNA gene of T. equi, four genotypes (A, C, D and E) were detected, whereas four B. caballi sequences clustered within a single genotype (A). A single dog infected with T. equi was found and confirmed by sequencing, clustering within genotype D. Our study further proved limited host specificity of piroplasmid. Higher prevalence was revealed inside the Danube Delta, presumably caused by different environmental conditions, different tick densities and lower of veterinary services. Rural areas of Romania, particularly the localities covering Danube Delta, are still not sufficiently explored in terms of epidemiological aspects, despite the large density of domestic animals living in close contact with people and natural environment of the Danube Delta Biosphere Reserve. Between 2010 and 2012, a survey on equine piroplasmids species was performed in this area, using a total of 178 horses, 15 donkeys and 177 dogs from 18 localities inside and outside the Danube Delta. None of the sampled hosts showed any clinical symptoms typical for equine piroplasmoses. A 25.4% overall prevalence for both equine species of piroplasmids was detected by PCR. Detection by %22catch-all%22 primers followed by multiplex PCR revealed 20.3% prevalence of T. equi, beside 2.2% of B. caballi and 3.0% of mixed infections for all examined animals. Based on sequencing of 67 PCR amplicons of 18S rRNA gene of T. equi, four genotypes (A, C, D and E) were detected, whereas four B. caballi sequences clustered within a single genotype (A). A single dog infected with T. equi was found and confirmed by sequencing, clustering within genotype D. Our study further proved limited host specificity of piroplasmid. Higher prevalence was revealed inside the Danube Delta, presumably caused by different environmental conditions, different tick densities and lower of veterinary services.
dcterms:title
Piroplasms in feral and domestic equines in rural areas of the Danube Delta, Romania, with survey of dogs as a possible reservoir Piroplasms in feral and domestic equines in rural areas of the Danube Delta, Romania, with survey of dogs as a possible reservoir
skos:prefLabel
Piroplasms in feral and domestic equines in rural areas of the Danube Delta, Romania, with survey of dogs as a possible reservoir Piroplasms in feral and domestic equines in rural areas of the Danube Delta, Romania, with survey of dogs as a possible reservoir
skos:notation
RIV/62157124:16810/14:43872648!RIV15-MSM-16810___
n4:aktivita
n6:I n6:P
n4:aktivity
I, P(ED1.1.00/02.0068)
n4:cisloPeriodika
3-4
n4:dodaniDat
n15:2015
n4:domaciTvurceVysledku
n8:2446391 n8:7286384
n4:druhVysledku
n11:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n9:predkladatel
n4:idSjednocenehoVysledku
36684
n4:idVysledku
RIV/62157124:16810/14:43872648
n4:jazykVysledku
n19:eng
n4:klicovaSlova
Genetic diversity; Danube Delta; Equine piroplasmoses; Babesia caballi; T. equi
n4:klicoveSlovo
n5:T.%20equi n5:Babesia%20caballi n5:Danube%20Delta n5:Equine%20piroplasmoses n5:Genetic%20diversity
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[FC2F505B8956]
n4:nazevZdroje
Veterinary Parasitology
n4:obor
n7:GJ
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
7
n4:projekt
n13:ED1.1.00%2F02.0068
n4:rokUplatneniVysledku
n15:2014
n4:svazekPeriodika
206
n4:tvurceVysledku
Petrželková, Klára J. Qablan, Moneeb Ahmad Mohannad D'Amico, Gianluca Mihalca, Andrei D. Oborník, Miroslav Modrý, David Gallusová, Martina
n4:wos
000347767000024
s:issn
0304-4017
s:numberOfPages
6
n10:doi
10.1016/j.vetpar.2014.10.018
n17:organizacniJednotka
16810