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Statements

Subject Item
n2:RIV%2F62157124%3A16810%2F13%3A43871517%21RIV14-MSM-16810___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Undercooked meat containing tissue cysts is one of the most common sources of Toxoplasma gondii infection in humans. Goats are very susceptible to clinical toxoplasmosis, and especially kids are common food animals, thereby representing a risk for human infection. A sequence-specific magnetic capture method was used for isolation of T. gondii DNA from tissue samples from experimentally infected goat-kids and real-time PCR for the 529 bp repeat element allowed quantification of T. gondii DNA. The contamination level in different types of tissue and in two groups of goats euthanized 30 and 90 dpi was compared. The highest concentration of T. gondii DNA in both groups of goats was found in lung tissue, but only the higher parasite count in lung tissue compared to other organs in group A (euthanized 30 dpi) was statistically significant. T. gondii concentrations were higher in liver and dorsal muscle samples from goats euthanized 90 dpi than in goats euthanized at 30 dpi, while the T. gondii concentration in hearts decreased. This study describes for the first time distribution of T. gondii parasites in post-weaned goat kids. New information about T. gondii predilection sites in goats and about the progression of infection between 30 and 90 dpi was achieved. Undercooked meat containing tissue cysts is one of the most common sources of Toxoplasma gondii infection in humans. Goats are very susceptible to clinical toxoplasmosis, and especially kids are common food animals, thereby representing a risk for human infection. A sequence-specific magnetic capture method was used for isolation of T. gondii DNA from tissue samples from experimentally infected goat-kids and real-time PCR for the 529 bp repeat element allowed quantification of T. gondii DNA. The contamination level in different types of tissue and in two groups of goats euthanized 30 and 90 dpi was compared. The highest concentration of T. gondii DNA in both groups of goats was found in lung tissue, but only the higher parasite count in lung tissue compared to other organs in group A (euthanized 30 dpi) was statistically significant. T. gondii concentrations were higher in liver and dorsal muscle samples from goats euthanized 90 dpi than in goats euthanized at 30 dpi, while the T. gondii concentration in hearts decreased. This study describes for the first time distribution of T. gondii parasites in post-weaned goat kids. New information about T. gondii predilection sites in goats and about the progression of infection between 30 and 90 dpi was achieved.
dcterms:title
Quantification of Toxoplasma gondii in tissue samples of experimentally infected goats by magnetic capture and real-time PCR Quantification of Toxoplasma gondii in tissue samples of experimentally infected goats by magnetic capture and real-time PCR
skos:prefLabel
Quantification of Toxoplasma gondii in tissue samples of experimentally infected goats by magnetic capture and real-time PCR Quantification of Toxoplasma gondii in tissue samples of experimentally infected goats by magnetic capture and real-time PCR
skos:notation
RIV/62157124:16810/13:43871517!RIV14-MSM-16810___
n15:predkladatel
n16:orjk%3A16810
n3:aktivita
n12:S n12:P
n3:aktivity
P(ED0006/01/01), P(ED1.1.00/02.0068), S
n3:cisloPeriodika
1-3
n3:dodaniDat
n17:2014
n3:domaciTvurceVysledku
n6:6048412
n3:druhVysledku
n9:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
101196
n3:idVysledku
RIV/62157124:16810/13:43871517
n3:jazykVysledku
n4:eng
n3:klicovaSlova
Real-time PCR; Magnetic capture; Experimentally infected goats; Toxoplasma gondii
n3:klicoveSlovo
n11:Experimentally%20infected%20goats n11:Toxoplasma%20gondii n11:Magnetic%20capture n11:Real-time%20PCR
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[A4EB481CBE3F]
n3:nazevZdroje
Veterinary Parasitology
n3:obor
n13:GJ
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
8
n3:projekt
n18:ED1.1.00%2F02.0068 n18:ED0006%2F01%2F01
n3:rokUplatneniVysledku
n17:2013
n3:svazekPeriodika
193
n3:tvurceVysledku
Opsteegh, Marieke Neumayerová, Helena Baláž, Vojtěch Juránková, Jana Frencová, Anita Koudela, Břetislav Volf, Jiří Kovařčík, Kamil
s:issn
0304-4017
s:numberOfPages
5
n10:doi
10.1016/j.vetpar.2012.11.016
n14:organizacniJednotka
16810