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Statements

Subject Item
n2:RIV%2F62157124%3A16810%2F11%3A43870480%21RIV12-GA0-16810___
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Aim of the study is to evaluate the use of recombinant Bhlp29.7 in immunoblotting with sera as a means to detect pig herds infected with Brachyspira hyodysenteriae. Methods and Results: Sera samples from 789 sows and rectal swabs from 838 pigs of various categories on 22 farms of different size (median 450 animals), production type and history of swine dysentery (SD) were examined. Sera from 378 sows from farms with previous SD history were examined via immunoblotting. Specific antibodies were detected in 79 of these (20,9%). Examination of 411 serum samples from sows and gilts taken on 11 farms without previous history of SD detected specific antibodies in 13 sows and gilts (3,2%). These 13, however, had come from farms where the presence of B. hyodysenteriae was confirmed or SD status was not known. Seroprevalence in herds with previous SD history ranged from 2,5 to 35,7%. B. hyodysenteriae was confirmed on six (27,3%) of 22 monitored farms. Conclusions: Immunoblotting using recombinant antigen Bhlp29.7 in conjunction with culturing B. hyodysenteriae proved to be a valuable tool for detecting swine herds latently infected with B. hyodysenteriae. Significance and Impact of the Study: The use of immunoblotting with recombinant Bhlp29.7 should prove to be a useful adjunct to detecting herds with SD, and hence, it will assist in controlling this important disease. Aim of the study is to evaluate the use of recombinant Bhlp29.7 in immunoblotting with sera as a means to detect pig herds infected with Brachyspira hyodysenteriae. Methods and Results: Sera samples from 789 sows and rectal swabs from 838 pigs of various categories on 22 farms of different size (median 450 animals), production type and history of swine dysentery (SD) were examined. Sera from 378 sows from farms with previous SD history were examined via immunoblotting. Specific antibodies were detected in 79 of these (20,9%). Examination of 411 serum samples from sows and gilts taken on 11 farms without previous history of SD detected specific antibodies in 13 sows and gilts (3,2%). These 13, however, had come from farms where the presence of B. hyodysenteriae was confirmed or SD status was not known. Seroprevalence in herds with previous SD history ranged from 2,5 to 35,7%. B. hyodysenteriae was confirmed on six (27,3%) of 22 monitored farms. Conclusions: Immunoblotting using recombinant antigen Bhlp29.7 in conjunction with culturing B. hyodysenteriae proved to be a valuable tool for detecting swine herds latently infected with B. hyodysenteriae. Significance and Impact of the Study: The use of immunoblotting with recombinant Bhlp29.7 should prove to be a useful adjunct to detecting herds with SD, and hence, it will assist in controlling this important disease.
dcterms:title
Evaluation of the use of recombinant Bhlp29.7 in immunoblotting with pig serum as a means to identify herds infected with Brachyspira hyodysenteriae Evaluation of the use of recombinant Bhlp29.7 in immunoblotting with pig serum as a means to identify herds infected with Brachyspira hyodysenteriae
skos:prefLabel
Evaluation of the use of recombinant Bhlp29.7 in immunoblotting with pig serum as a means to identify herds infected with Brachyspira hyodysenteriae Evaluation of the use of recombinant Bhlp29.7 in immunoblotting with pig serum as a means to identify herds infected with Brachyspira hyodysenteriae
skos:notation
RIV/62157124:16810/11:43870480!RIV12-GA0-16810___
n5:predkladatel
n12:orjk%3A16810
n3:aktivita
n18:P
n3:aktivity
P(GA524/06/1501)
n3:cisloPeriodika
4
n3:dodaniDat
n9:2012
n3:domaciTvurceVysledku
n10:8180458 n10:2497417
n3:druhVysledku
n15:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
198402
n3:idVysledku
RIV/62157124:16810/11:43870480
n3:jazykVysledku
n20:eng
n3:klicovaSlova
Brachyspira hyodysenteriae; recombinant protein; Pig
n3:klicoveSlovo
n11:recombinant%20protein n11:Brachyspira%20hyodysenteriae n11:Pig
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[6F306C68D449]
n3:nazevZdroje
Letters in Applied Microbiology
n3:obor
n17:GJ
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n16:GA524%2F06%2F1501
n3:rokUplatneniVysledku
n9:2011
n3:svazekPeriodika
53
n3:tvurceVysledku
Čížek, Alois Celer, Vladimír Prášek, Josef Lobová, Dana
n3:wos
000295095600012
s:issn
0266-8254
s:numberOfPages
7
n14:doi
10.1111/j.1472-765X.2011.03134.x
n19:organizacniJednotka
16810