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Statements

Subject Item
n2:RIV%2F62157124%3A16370%2F13%3A43871830%21RIV14-MSM-16370___
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Kinetin (N6-furfuryladenine) belongs to a group of plant growth hormones involved in cell division, differentiation and other physiological processes. One of the possible ways to obtain biologically active compounds is to complex biologically relevant natural compounds to suitable metal atoms. In this work, two structural groups of Zn(II) complexes [Zn(L-n)(2Cl)-2] center dot Solv (1-5) and [Zn(HLn))Cl-3] center dot xL(n) (6-7); n=1-5, Solv=CH3OH for 1 and 2H2O for 2; x =1 for 6 and 2 for 7; involving a phytohormone kinetin and its derivatives (L-n) were evaluated for their ability to modulate secretion of tumour necrosis factor (TNF)-alfa, interleukin (IL)-1 beta and matrix metalloproteinase (MMP)-2 in a lipopolysaccharide (LPS)-activated macrophage-like THP-1 cell model. The penetration of the complexes to cells was also detected. The mechanism of interactions of the zinc(II) complexes with a fluorescent sensor N-(6-methoxy-8-quinolyl)-p-toluene sulphonamide (TSQ) and sulfur-containing biomolecules (l-cysteine and reduced glutathione) was studied by electrospray-ionization mass spectrometry and flow-injection analysis with fluorescence detection. The present study showed that the tested complexes exhibited a low cytotoxic effect on the THP-1 cell line (IC50}40 uM), apart from complex 4, with an IC50=10.9 mikroM. Regarding the inflammation-related processes, the Zn(II) complexes decreased IL-1 beta production by a factor of 1.47-2.22 compared with the control (DMSO), but did not affect TNF-alfa and MMP-2 secretions. However, application of the Zn(II) complexes noticeably changed the pro-MMP-2/MMP-2 ratio towards a higher amount of maturated MMP-2, when they induced a 4-times higher production of maturated MMP-2 in comparison with the vehicle-treated cells under LPS stimulation. These results indicated that the complexes are able to modulate an inflammatory response by influencing secretion and activity of several inflammation-related cytokines and enzymes. Kinetin (N6-furfuryladenine) belongs to a group of plant growth hormones involved in cell division, differentiation and other physiological processes. One of the possible ways to obtain biologically active compounds is to complex biologically relevant natural compounds to suitable metal atoms. In this work, two structural groups of Zn(II) complexes [Zn(L-n)(2Cl)-2] center dot Solv (1-5) and [Zn(HLn))Cl-3] center dot xL(n) (6-7); n=1-5, Solv=CH3OH for 1 and 2H2O for 2; x =1 for 6 and 2 for 7; involving a phytohormone kinetin and its derivatives (L-n) were evaluated for their ability to modulate secretion of tumour necrosis factor (TNF)-alfa, interleukin (IL)-1 beta and matrix metalloproteinase (MMP)-2 in a lipopolysaccharide (LPS)-activated macrophage-like THP-1 cell model. The penetration of the complexes to cells was also detected. The mechanism of interactions of the zinc(II) complexes with a fluorescent sensor N-(6-methoxy-8-quinolyl)-p-toluene sulphonamide (TSQ) and sulfur-containing biomolecules (l-cysteine and reduced glutathione) was studied by electrospray-ionization mass spectrometry and flow-injection analysis with fluorescence detection. The present study showed that the tested complexes exhibited a low cytotoxic effect on the THP-1 cell line (IC50}40 uM), apart from complex 4, with an IC50=10.9 mikroM. Regarding the inflammation-related processes, the Zn(II) complexes decreased IL-1 beta production by a factor of 1.47-2.22 compared with the control (DMSO), but did not affect TNF-alfa and MMP-2 secretions. However, application of the Zn(II) complexes noticeably changed the pro-MMP-2/MMP-2 ratio towards a higher amount of maturated MMP-2, when they induced a 4-times higher production of maturated MMP-2 in comparison with the vehicle-treated cells under LPS stimulation. These results indicated that the complexes are able to modulate an inflammatory response by influencing secretion and activity of several inflammation-related cytokines and enzymes.
dcterms:title
Zn(II)-Chlorido complexes of phytohormone kinetin and its derivatives modulate expression of inflammatory mediators in THP-1 cells. Zn(II)-Chlorido complexes of phytohormone kinetin and its derivatives modulate expression of inflammatory mediators in THP-1 cells.
skos:prefLabel
Zn(II)-Chlorido complexes of phytohormone kinetin and its derivatives modulate expression of inflammatory mediators in THP-1 cells. Zn(II)-Chlorido complexes of phytohormone kinetin and its derivatives modulate expression of inflammatory mediators in THP-1 cells.
skos:notation
RIV/62157124:16370/13:43871830!RIV14-MSM-16370___
n3:predkladatel
n16:orjk%3A16370
n4:aktivita
n11:I n11:S n11:P
n4:aktivity
I, P(ED2.1.00/03.0058), P(EE2.3.20.0017), S
n4:cisloPeriodika
6
n4:dodaniDat
n6:2014
n4:domaciTvurceVysledku
n19:9600426
n4:druhVysledku
n15:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
119139
n4:idVysledku
RIV/62157124:16370/13:43871830
n4:jazykVysledku
n20:eng
n4:klicovaSlova
TNF-alfa; THP-1; inflammatory mediators; kinetin; ZnII-Chlorido complexes
n4:klicoveSlovo
n5:THP-1 n5:inflammatory%20mediators n5:TNF-alfa n5:kinetin n5:ZnII-Chlorido%20complexes
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[DF45ADFC8F21]
n4:nazevZdroje
PLoS ONE
n4:obor
n18:FR
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
5
n4:projekt
n14:EE2.3.20.0017 n14:ED2.1.00%2F03.0058
n4:rokUplatneniVysledku
n6:2013
n4:svazekPeriodika
8
n4:tvurceVysledku
Vančo, Ján Novotná, Radka Hošek, Jan Trávníček, Zdeněk Babula, Petr
n4:wos
000319872300058
s:issn
1932-6203
s:numberOfPages
10
n13:doi
10.1371/journal.pone.0065214
n7:organizacniJednotka
16370