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Statements

Subject Item
n2:RIV%2F62157124%3A16370%2F10%3A00002664%21RIV11-MSM-16370___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Calibration based on the %22single-point calibration method%22, a simple exponential transformation of the response function of an evaporative light scattering detector was improved and applied to analysis of selected saccharides under hydrophilic interaction chromatography mode (a polar phase LiChrospher100 DIOL, mobile phase acetonitrile/water). The improved approach to the calibration procedure yielded a calibration curve with an excellent linearity (quality coefficient <5%). This quantitative evaluation of chromatograms of D-galactose suggested that not only anomers but even pyranose and furanose forms of the anomers could be resolved the resulting calculations of abundance of the anomeric form strongly correlated with data from the literature obtained mostly by NMR studies (analogous results were also obtained for D-arabinose, D-glucose, and D-mannose). Because of the rapid separation (retention time less than 10 min), the observed correlation enabled to monitor anomeric conversion (muta Calibration based on the %22single-point calibration method%22, a simple exponential transformation of the response function of an evaporative light scattering detector was improved and applied to analysis of selected saccharides under hydrophilic interaction chromatography mode (a polar phase LiChrospher100 DIOL, mobile phase acetonitrile/water). The improved approach to the calibration procedure yielded a calibration curve with an excellent linearity (quality coefficient <5%). This quantitative evaluation of chromatograms of D-galactose suggested that not only anomers but even pyranose and furanose forms of the anomers could be resolved the resulting calculations of abundance of the anomeric form strongly correlated with data from the literature obtained mostly by NMR studies (analogous results were also obtained for D-arabinose, D-glucose, and D-mannose). Because of the rapid separation (retention time less than 10 min), the observed correlation enabled to monitor anomeric conversion (muta
dcterms:title
Monitoring of mutarotation of monosaccharides by hydrophilic interaction chromatography Monitoring of mutarotation of monosaccharides by hydrophilic interaction chromatography
skos:prefLabel
Monitoring of mutarotation of monosaccharides by hydrophilic interaction chromatography Monitoring of mutarotation of monosaccharides by hydrophilic interaction chromatography
skos:notation
RIV/62157124:16370/10:00002664!RIV11-MSM-16370___
n3:aktivita
n16:V
n3:aktivity
V
n3:cisloPeriodika
6-7
n3:dodaniDat
n8:2011
n3:domaciTvurceVysledku
n4:1902555
n3:druhVysledku
n7:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
272485
n3:idVysledku
RIV/62157124:16370/10:00002664
n3:jazykVysledku
n11:eng
n3:klicovaSlova
chromatography; sacharides; monosacharides
n3:klicoveSlovo
n5:monosacharides n5:chromatography n5:sacharides
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[0A1AD45B41D7]
n3:nazevZdroje
Journal of Separation Science
n3:obor
n13:FR
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
1
n3:rokUplatneniVysledku
n8:2010
n3:svazekPeriodika
33
n3:tvurceVysledku
Pazourek, Jiří
n3:wos
000276782700032
s:issn
1615-9306
s:numberOfPages
8
n12:organizacniJednotka
16370