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Statements

Subject Item
n2:RIV%2F62157124%3A16270%2F13%3A43873338%21RIV15-MSM-16270___
rdf:type
skos:Concept n12:Vysledek
dcterms:description
The conventional polymerase chain reaction (PCR) method to detect the major allergenic protein parvalbumin beta 2 of Atlantic herring (Clupea harengus) and Pacific herring (Clupea pallasii) was developed. The specific set of primers for the amplification of the partial genomic sequence of the pvalb 2 gene encoding the main fish allergen of both herrings was designed and applied to the investigation of 24 commercial fish products. The targeted amplicon size was 189bp of pvalb 2 gene of Atlantic herring and Pacific herring. As the internal amplification control, the DNA of 18S rRNA gene for eukaryotes (141bp) was successfully used. The specificity of designed primer pair using 26 various fish species was assessed. The intrinsic detection limit was 10pg mu l(-1) of the present specific DNA. Atlantic herring or Pacific herring allergenic parvalbumins were detected in 22 investigated fish products in conformity with the package declaration. Two fish products were negative in spite of the declaration. The proposed PCR method is specific enough and can be used for the detection of Atlantic and Pacific herrings' major allergen parvalbumin beta 2 in fish food products. The conventional polymerase chain reaction (PCR) method to detect the major allergenic protein parvalbumin beta 2 of Atlantic herring (Clupea harengus) and Pacific herring (Clupea pallasii) was developed. The specific set of primers for the amplification of the partial genomic sequence of the pvalb 2 gene encoding the main fish allergen of both herrings was designed and applied to the investigation of 24 commercial fish products. The targeted amplicon size was 189bp of pvalb 2 gene of Atlantic herring and Pacific herring. As the internal amplification control, the DNA of 18S rRNA gene for eukaryotes (141bp) was successfully used. The specificity of designed primer pair using 26 various fish species was assessed. The intrinsic detection limit was 10pg mu l(-1) of the present specific DNA. Atlantic herring or Pacific herring allergenic parvalbumins were detected in 22 investigated fish products in conformity with the package declaration. Two fish products were negative in spite of the declaration. The proposed PCR method is specific enough and can be used for the detection of Atlantic and Pacific herrings' major allergen parvalbumin beta 2 in fish food products.
dcterms:title
Detection of allergenic parvalbumin of Atlantic and Pacific herrings in fish products by PCR Detection of allergenic parvalbumin of Atlantic and Pacific herrings in fish products by PCR
skos:prefLabel
Detection of allergenic parvalbumin of Atlantic and Pacific herrings in fish products by PCR Detection of allergenic parvalbumin of Atlantic and Pacific herrings in fish products by PCR
skos:notation
RIV/62157124:16270/13:43873338!RIV15-MSM-16270___
n4:aktivita
n9:Z n9:I
n4:aktivity
I, Z(MZE0002716202)
n4:cisloPeriodika
10
n4:dodaniDat
n8:2015
n4:domaciTvurceVysledku
n16:5162823
n4:druhVysledku
n18:J
n4:duvernostUdaju
n6:S
n4:entitaPredkladatele
n15:predkladatel
n4:idSjednocenehoVysledku
68721
n4:idVysledku
RIV/62157124:16270/13:43873338
n4:jazykVysledku
n14:eng
n4:klicovaSlova
PCR; canned products; food allergens; DNA; fish allergens
n4:klicoveSlovo
n5:fish%20allergens n5:canned%20products n5:DNA n5:PCR n5:food%20allergens
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[A366770DA852]
n4:nazevZdroje
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
n4:obor
n13:GM
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
3
n4:rokUplatneniVysledku
n8:2013
n4:svazekPeriodika
30
n4:tvurceVysledku
Renčová, Eva Tremlová, Bohuslava Kostelníková, Darina
n4:wos
000324903700001
n4:zamer
n19:MZE0002716202
s:issn
1944-0049
s:numberOfPages
5
n17:doi
10.1080/19440049.2013.817024
n11:organizacniJednotka
16270