This HTML5 document contains 49 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n13http://localhost/temp/predkladatel/
n18http://linked.opendata.cz/resource/domain/vavai/projekt/
n6http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n19http://linked.opendata.cz/resource/domain/vavai/subjekt/
n11http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n20http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F62157124%3A16270%2F13%3A43872442%21RIV14-GA0-16270___/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
n5http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n8http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n16http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n10http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n14http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F62157124%3A16270%2F13%3A43872442%21RIV14-GA0-16270___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
We have performed a survey of Babesia canis prevalence within group of dogs living in Southern and Western Slovakia. Blood samples and sera from 217 dogs, including individuals suspected of having babesiosis, were examined by nested PCR-RFLP, light microscopy and indirect fluorescence antibody test (IFAT). The detection of B. canis DNA revealed the highest number of infected dogs in the region of Nave Zamky, with 23 B. canis-Positive blood samples (35.4%, n = 65), followed by an area close to Komarno (both areas of Southern Slovakia), where 1 dog out of 52 collected (1.9%) had detectible B. canis DNA in the blood stream. The serological method revealed an opposing pattern, with only 3 dogs (4.8%, n = 63) sampled at Nove Zamky presenting IgG antibodies against B. canis, while in Komarno region such antibodies were detected in 15 dogs (28.8%, n = 52). This discrepancy may be because the majority of samples from Nove Zamky were dogs suspected of an acute phase of canine babesiosis, whereas dogs at Komarno were sampled during a vaccination campaign, and thus were without any clinical signs of the disease. The latter group contains evidently recovered carriers of IgG against B. canis. Hence, the combination of PCR-based and serological methods enabled us to discover both recently infected as well as recovered dogs, thus obtaining a more realistic view on the epidemiological situation. Remarkably, we did not find any positive sample in the vicinity of Stupava (district Malacky, Western Slovakia), either by PCR-RFLP, microscopy or IFAT (n=100). Considering the numerous falsely diagnosed cases of canine babesiosis, we suggest that light microscopy as the simplest and most accessible diagnostic test. Southern Slovakia was confirmed as an area of high risk of canine babesiosis, whereas conclusions about B. canis spreading over Western Slovakia should be considered with wariness. We have performed a survey of Babesia canis prevalence within group of dogs living in Southern and Western Slovakia. Blood samples and sera from 217 dogs, including individuals suspected of having babesiosis, were examined by nested PCR-RFLP, light microscopy and indirect fluorescence antibody test (IFAT). The detection of B. canis DNA revealed the highest number of infected dogs in the region of Nave Zamky, with 23 B. canis-Positive blood samples (35.4%, n = 65), followed by an area close to Komarno (both areas of Southern Slovakia), where 1 dog out of 52 collected (1.9%) had detectible B. canis DNA in the blood stream. The serological method revealed an opposing pattern, with only 3 dogs (4.8%, n = 63) sampled at Nove Zamky presenting IgG antibodies against B. canis, while in Komarno region such antibodies were detected in 15 dogs (28.8%, n = 52). This discrepancy may be because the majority of samples from Nove Zamky were dogs suspected of an acute phase of canine babesiosis, whereas dogs at Komarno were sampled during a vaccination campaign, and thus were without any clinical signs of the disease. The latter group contains evidently recovered carriers of IgG against B. canis. Hence, the combination of PCR-based and serological methods enabled us to discover both recently infected as well as recovered dogs, thus obtaining a more realistic view on the epidemiological situation. Remarkably, we did not find any positive sample in the vicinity of Stupava (district Malacky, Western Slovakia), either by PCR-RFLP, microscopy or IFAT (n=100). Considering the numerous falsely diagnosed cases of canine babesiosis, we suggest that light microscopy as the simplest and most accessible diagnostic test. Southern Slovakia was confirmed as an area of high risk of canine babesiosis, whereas conclusions about B. canis spreading over Western Slovakia should be considered with wariness.
dcterms:title
Conflicting results of serological, PCR and microscopic methods clarify the various risk levels of canine babesiosis in Slovakia: A complex approach to Babesia canis diagnostics Conflicting results of serological, PCR and microscopic methods clarify the various risk levels of canine babesiosis in Slovakia: A complex approach to Babesia canis diagnostics
skos:prefLabel
Conflicting results of serological, PCR and microscopic methods clarify the various risk levels of canine babesiosis in Slovakia: A complex approach to Babesia canis diagnostics Conflicting results of serological, PCR and microscopic methods clarify the various risk levels of canine babesiosis in Slovakia: A complex approach to Babesia canis diagnostics
skos:notation
RIV/62157124:16270/13:43872442!RIV14-GA0-16270___
n11:predkladatel
n19:orjk%3A16270
n4:aktivita
n10:P
n4:aktivity
P(ED1.1.00/02.0068), P(GA524/09/0715)
n4:cisloPeriodika
3-4
n4:dodaniDat
n14:2014
n4:domaciTvurceVysledku
n6:5226104 n6:9081232
n4:druhVysledku
n12:J
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n20:predkladatel
n4:idSjednocenehoVysledku
66716
n4:idVysledku
RIV/62157124:16270/13:43872442
n4:jazykVysledku
n9:eng
n4:klicovaSlova
Microscopy; PCR-RFLP; IFAT; Diagnosis; Epidemiology; Tick-borne diseases; Babesia
n4:klicoveSlovo
n8:PCR-RFLP n8:Diagnosis n8:Tick-borne%20diseases n8:Babesia n8:Epidemiology n8:Microscopy n8:IFAT
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[A95E8773E26D]
n4:nazevZdroje
Veterinary Parasitology
n4:obor
n17:GJ
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
4
n4:projekt
n18:GA524%2F09%2F0715 n18:ED1.1.00%2F02.0068
n4:rokUplatneniVysledku
n14:2013
n4:svazekPeriodika
191
n4:tvurceVysledku
Panev, Aleksandar Sedlák, Kamil Široký, Pavel Kubelová, Michaela
n4:wos
000314073200017
s:issn
0304-4017
s:numberOfPages
5
n5:doi
10.1016/j.vetpar.2012.09.016
n13:organizacniJednotka
16270