This HTML5 document contains 63 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n14http://localhost/temp/predkladatel/
n17http://linked.opendata.cz/resource/domain/vavai/projekt/
n5http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n20http://linked.opendata.cz/resource/domain/vavai/subjekt/
n12http://linked.opendata.cz/ontology/domain/vavai/
n16http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
rdfshttp://www.w3.org/2000/01/rdf-schema#
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n7http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n8http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n11http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F62157124%3A16270%2F12%3A43871393%21RIV13-MSM-16270___/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n19http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n13http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n10http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F62157124%3A16270%2F12%3A43871393%21RIV13-MSM-16270___
rdf:type
n12:Vysledek skos:Concept
rdfs:seeAlso
http://www.electrochemsci.org/papers/vol7/7020943.pdf
dcterms:description
Lead(II) ions represent still threat to living organisms due to high burden of these ions in environment. The aim of this study was to design and perform experiments studying the interaction of lead(II) ions and proteins contained in egg albumen. For this purpose, we used electrochemical and electrophoretic techniques. We focused on a very detailed study of the interaction of lead(II) ions with the egg albumen proteins. Firstly, egg albumen was diluted with acetate buffer in the following ratios buffer: albumen - 1:0.5; 1: 1; 1: 2 and 1: 4. Additions of different concentrations of lead(II) ions into the diluted egg albumen were 0; 1; 5; 10; 25; 50; 100; 150; 200; 250; 500; 750 and 1,000 nM. Mixtures were then placed on thermoblock at 37 degrees C for 15, 30, 45 and 60 min. Based on determined protein profiles (as slopes of signal changes depending on the applied lead(II) concentration) at various dilutions it is clearly evident that the most significant changes are detected at ovotransferrin, avidin, lysozyme and ovomucoid. In addition, we employed optimized methods for studying of lead(II) induced complexes in died embryo of vulture. Contents of lead(II) ions, reduced and oxidised glutathione, metallothionein and zinc(II) ions were determined in albumen, yolk, liver, kidney, brain and bone obtained from the embryo. Lead(II) ions represent still threat to living organisms due to high burden of these ions in environment. The aim of this study was to design and perform experiments studying the interaction of lead(II) ions and proteins contained in egg albumen. For this purpose, we used electrochemical and electrophoretic techniques. We focused on a very detailed study of the interaction of lead(II) ions with the egg albumen proteins. Firstly, egg albumen was diluted with acetate buffer in the following ratios buffer: albumen - 1:0.5; 1: 1; 1: 2 and 1: 4. Additions of different concentrations of lead(II) ions into the diluted egg albumen were 0; 1; 5; 10; 25; 50; 100; 150; 200; 250; 500; 750 and 1,000 nM. Mixtures were then placed on thermoblock at 37 degrees C for 15, 30, 45 and 60 min. Based on determined protein profiles (as slopes of signal changes depending on the applied lead(II) concentration) at various dilutions it is clearly evident that the most significant changes are detected at ovotransferrin, avidin, lysozyme and ovomucoid. In addition, we employed optimized methods for studying of lead(II) induced complexes in died embryo of vulture. Contents of lead(II) ions, reduced and oxidised glutathione, metallothionein and zinc(II) ions were determined in albumen, yolk, liver, kidney, brain and bone obtained from the embryo.
dcterms:title
Metallomics Study of Lead-Protein Interactions in Albumen by Electrochemical and Electrophoretic Methods Metallomics Study of Lead-Protein Interactions in Albumen by Electrochemical and Electrophoretic Methods
skos:prefLabel
Metallomics Study of Lead-Protein Interactions in Albumen by Electrochemical and Electrophoretic Methods Metallomics Study of Lead-Protein Interactions in Albumen by Electrochemical and Electrophoretic Methods
skos:notation
RIV/62157124:16270/12:43871393!RIV13-MSM-16270___
n12:predkladatel
n20:orjk%3A16270
n4:aktivita
n9:V n9:Z n9:P
n4:aktivity
P(ED1.1.00/02.0068), P(GA102/08/1546), V, Z(MSM0021622412)
n4:cisloPeriodika
2
n4:dodaniDat
n10:2013
n4:domaciTvurceVysledku
n5:4771079 n5:9847065
n4:druhVysledku
n19:J
n4:duvernostUdaju
n8:S
n4:entitaPredkladatele
n11:predkladatel
n4:idSjednocenehoVysledku
149646
n4:idVysledku
RIV/62157124:16270/12:43871393
n4:jazykVysledku
n18:eng
n4:klicovaSlova
proteomics; spectrophotometry; metallothionein; capillary electrophoresis; heavy metal; differential pulse voltammetry; automated electrochemical detection; protein interaction
n4:klicoveSlovo
n7:protein%20interaction n7:metallothionein n7:heavy%20metal n7:differential%20pulse%20voltammetry n7:automated%20electrochemical%20detection n7:proteomics n7:capillary%20electrophoresis n7:spectrophotometry
n4:kodStatuVydavatele
RS - Srbská republika
n4:kontrolniKodProRIV
[FFF6569C72CC]
n4:nazevZdroje
International journal of electrochemical science
n4:obor
n13:EB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
14
n4:projekt
n17:ED1.1.00%2F02.0068 n17:GA102%2F08%2F1546
n4:rokUplatneniVysledku
n10:2012
n4:svazekPeriodika
7
n4:tvurceVysledku
Křížková, Soňa Kizek, René Pohanka, Miroslav Krejčová, Ludmila Trnková, Libuše Beklová, Miroslava Hájková, Pavlína Sochor, Jiří Vrba, Radimír Pikula, Jiří Hynek, David Ruttkay-Nedecký, Branislav Adam, Vojtěch Hubálek, Jaromír
n4:wos
000302730000004
n4:zamer
n16:MSM0021622412
s:issn
1452-3981
s:numberOfPages
22
n14:organizacniJednotka
16270