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Statements

Subject Item
n2:RIV%2F62157124%3A16270%2F11%3A43870927%21RIV12-GA0-16270___
rdf:type
n9:Vysledek skos:Concept
dcterms:description
The aim of this study was to analyze the occurrence of extended-spectrum beta-lactamase (ESBL)- and plasmid-mediated quinolone resistance (PMQR)-positive Escherichia coli isolated from cormorants (Phalacrocorax carbo) and mallards (Anas platyrhynchos) in the central Europe, and to determine the importance of these birds in spreading of these bacteria. The samples were cultivated parallelly on MacConkey agar (MCA) with cefotaxime (2 mg/L) to isolate ESBL-producing E. coli and MCA with ciprofloxacin (0.05 mg/L) to isolate fluoroquinolone-resistant E. coli. PCR was used to detect specific antibiotic resistance genes. Plasmids carrying ESBL and/or PMQR genes were transferred by conjugation or transformation and characterized by PCR-based replicon typing and restriction analysis. From waterbirds, ten ESBL-producing E. coli with the blaCTX-M-15 or blaCTX-M-27 genes were isolated. In most isolates with blaCTX-M-15, the aac(6')Ib-cr gene was also detected. The bla genes were harbored by F and I1 conjugative plasmids. PMQR genes were detected in 25 E. coli. Six strains carried aac(6')Ib-cr and nineteen strains were positive for qnrS1. The genes were located on transferable plasmids of incompatibility groups N or X2. The finding of CTX-M-type ESBLs and PMQR genes in these animal species indicates rapid spread of bacteria with these resistance genes in the environment. The aim of this study was to analyze the occurrence of extended-spectrum beta-lactamase (ESBL)- and plasmid-mediated quinolone resistance (PMQR)-positive Escherichia coli isolated from cormorants (Phalacrocorax carbo) and mallards (Anas platyrhynchos) in the central Europe, and to determine the importance of these birds in spreading of these bacteria. The samples were cultivated parallelly on MacConkey agar (MCA) with cefotaxime (2 mg/L) to isolate ESBL-producing E. coli and MCA with ciprofloxacin (0.05 mg/L) to isolate fluoroquinolone-resistant E. coli. PCR was used to detect specific antibiotic resistance genes. Plasmids carrying ESBL and/or PMQR genes were transferred by conjugation or transformation and characterized by PCR-based replicon typing and restriction analysis. From waterbirds, ten ESBL-producing E. coli with the blaCTX-M-15 or blaCTX-M-27 genes were isolated. In most isolates with blaCTX-M-15, the aac(6')Ib-cr gene was also detected. The bla genes were harbored by F and I1 conjugative plasmids. PMQR genes were detected in 25 E. coli. Six strains carried aac(6')Ib-cr and nineteen strains were positive for qnrS1. The genes were located on transferable plasmids of incompatibility groups N or X2. The finding of CTX-M-type ESBLs and PMQR genes in these animal species indicates rapid spread of bacteria with these resistance genes in the environment.
dcterms:title
Colonization of wild waterbirds by cephalosporins and fluoroquinolone resistant Escherichia coli strains. Colonization of wild waterbirds by cephalosporins and fluoroquinolone resistant Escherichia coli strains.
skos:prefLabel
Colonization of wild waterbirds by cephalosporins and fluoroquinolone resistant Escherichia coli strains. Colonization of wild waterbirds by cephalosporins and fluoroquinolone resistant Escherichia coli strains.
skos:notation
RIV/62157124:16270/11:43870927!RIV12-GA0-16270___
n9:predkladatel
n20:orjk%3A16270
n3:aktivita
n8:Z n8:P
n3:aktivity
P(GPP502/10/P083), Z(MSM6215712402)
n3:dodaniDat
n16:2012
n3:domaciTvurceVysledku
n14:8073236 n14:7724896 n14:6073816
n3:druhVysledku
n19:D
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n22:predkladatel
n3:idSjednocenehoVysledku
190843
n3:idVysledku
RIV/62157124:16270/11:43870927
n3:jazykVysledku
n17:eng
n3:klicovaSlova
resistance; PMQR genes; extended-spectrum beta-lactamases; waterbirds; Escherichia coli
n3:klicoveSlovo
n13:PMQR%20genes n13:Escherichia%20coli n13:extended-spectrum%20beta-lactamases n13:resistance n13:waterbirds
n3:kontrolniKodProRIV
[24F74CBA3AAC]
n3:mistoKonaniAkce
Brno
n3:mistoVydani
Brno
n3:nazevZdroje
Sborník XIII. konference mladých vědeckých pracovníků s mezinárodní účastí
n3:obor
n5:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
7
n3:projekt
n21:GPP502%2F10%2FP083
n3:rokUplatneniVysledku
n16:2011
n3:tvurceVysledku
Hrušáková, Jolana Hanušová, Ladislava Svoboda, Ondřej Čížek, Alois Literák, Ivan Janoszowská, Dagmar Dolejská, Monika
n3:typAkce
n11:EUR
n3:zahajeniAkce
2011-06-01+02:00
n3:zamer
n4:MSM6215712402
s:numberOfPages
3
n23:hasPublisher
Veterinární a farmaceutická univerzita Brno
n6:isbn
978-80-7305-010-8
n10:organizacniJednotka
16270