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Statements

Subject Item
n2:RIV%2F62157124%3A16270%2F09%3A00002377%21RIV10-MSM-16270___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Cholesterol concentrations in goats milk, goats milk cheeses, ewes milk, ewes milk cheeses, dairy bio-products, and concentrations of cholesterol, stigmasterol and sitosterol in butter, butter with added vegetable fats and margarines were evaluated by RP HPLC method. Parallel analyses by capillary GC were performed. Prior to the final chromatographic analyses the saponification step with methanolic potassium hydroxide solution was used, followed by the extraction of the unsaponificable residue into n-hexane. Parameters of RP HPLC method were compared with parameters of GC determination The detection limits (LOD) determinated on the bases of blank samples analysis were 5.2 mg.kg-1 for cholesterol, 4.8 mg.kg-1 for stigmasterol and 14.7 mg.kg-1 for sitosterol. Recovery ranged between 80 - 92%, repeatibility expressed as RSD of 12 paralel samples measurements was 4.2 - 6.8%. Accuracy tested on the SRM 1845 Whole Egg Powder (NIST) was 95.7 % Cholesterol concentrations in goats milk, goats milk cheeses, ewes milk, ewes milk cheeses, dairy bio-products, and concentrations of cholesterol, stigmasterol and sitosterol in butter, butter with added vegetable fats and margarines were evaluated by RP HPLC method. Parallel analyses by capillary GC were performed. Prior to the final chromatographic analyses the saponification step with methanolic potassium hydroxide solution was used, followed by the extraction of the unsaponificable residue into n-hexane. Parameters of RP HPLC method were compared with parameters of GC determination The detection limits (LOD) determinated on the bases of blank samples analysis were 5.2 mg.kg-1 for cholesterol, 4.8 mg.kg-1 for stigmasterol and 14.7 mg.kg-1 for sitosterol. Recovery ranged between 80 - 92%, repeatibility expressed as RSD of 12 paralel samples measurements was 4.2 - 6.8%. Accuracy tested on the SRM 1845 Whole Egg Powder (NIST) was 95.7 %
dcterms:title
Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC Methods. Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC Methods.
skos:prefLabel
Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC Methods. Determination of Sterols in Dairy Products and Vegetable Fats by HPLC and GC Methods.
skos:notation
RIV/62157124:16270/09:00002377!RIV10-MSM-16270___
n3:aktivita
n18:Z
n3:aktivity
Z(MSM6215712402)
n3:cisloPeriodika
1
n3:dodaniDat
n6:2010
n3:domaciTvurceVysledku
n8:4339495 n8:5739446 n8:3806669 n8:6796133
n3:druhVysledku
n15:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
309941
n3:idVysledku
RIV/62157124:16270/09:00002377
n3:jazykVysledku
n13:eng
n3:klicovaSlova
cholesterol; sterols; milk; dairy products; RP HPLC; GC
n3:klicoveSlovo
n5:sterols n5:cholesterol n5:RP%20HPLC n5:GC n5:milk n5:dairy%20products
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[9886FEBA27D1]
n3:nazevZdroje
Czech Journal of Food Science
n3:obor
n10:GM
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:rokUplatneniVysledku
n6:2009
n3:svazekPeriodika
27
n3:tvurceVysledku
Dračková, Michaela Janoušková, Eva Vorlová, Lenka Janštová, Bohumíra Borkovcová, Ivana
n3:wos
000269005600068
n3:zamer
n4:MSM6215712402
s:issn
1212-1800
s:numberOfPages
3
n16:organizacniJednotka
16270