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Statements

Subject Item
n2:RIV%2F62157124%3A16170%2F14%3A43873294%21RIV15-MSM-16170___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Toxoplasma gondii is a zoonotic parasite with a world-wide distribution. House mice (Mus musculus) play an important role as a reservoir host in the parasite life cycle. However, their detection in mouse brain is limited because the host potentially harbours only a few tissue cysts. In order to improve the diagnosis, we tested a novel protocol for T. gondii detection in mice and compared this technique to a standard PCR-based protocol using a commercial kit for DNA isolation. Efficacy of magnetic capture for isolation of T. gondii DNA from whole host brains was tested in brain samples of laboratory mice spiked with 1 up to 104 tachyzoites. Real-time PCR revealed that even 1-5 tachyzoites can be detected after magnetic capture. Also this method is suitable to quantify parasite numbers in mouse brains with more than 10 tachyzoite equivalents. To assess the two techniques in wild mice, we employed a dataset consisting of 243 individuals. The prevalence of T. gondii detected by magnetic capture and qPCR and by commercial isolation and PCR was 1.2% and 0%, respectively. The magnetic capture and quantitative PCR seems to be a highly sensitive and specific diagnostic method for both laboratory research and wild population surveys. Toxoplasma gondii is a zoonotic parasite with a world-wide distribution. House mice (Mus musculus) play an important role as a reservoir host in the parasite life cycle. However, their detection in mouse brain is limited because the host potentially harbours only a few tissue cysts. In order to improve the diagnosis, we tested a novel protocol for T. gondii detection in mice and compared this technique to a standard PCR-based protocol using a commercial kit for DNA isolation. Efficacy of magnetic capture for isolation of T. gondii DNA from whole host brains was tested in brain samples of laboratory mice spiked with 1 up to 104 tachyzoites. Real-time PCR revealed that even 1-5 tachyzoites can be detected after magnetic capture. Also this method is suitable to quantify parasite numbers in mouse brains with more than 10 tachyzoite equivalents. To assess the two techniques in wild mice, we employed a dataset consisting of 243 individuals. The prevalence of T. gondii detected by magnetic capture and qPCR and by commercial isolation and PCR was 1.2% and 0%, respectively. The magnetic capture and quantitative PCR seems to be a highly sensitive and specific diagnostic method for both laboratory research and wild population surveys.
dcterms:title
Efficacy of magnetic capture in comparison with conventional DNA isolation in a survey of Toxoplasma gondii in wild house mice Efficacy of magnetic capture in comparison with conventional DNA isolation in a survey of Toxoplasma gondii in wild house mice
skos:prefLabel
Efficacy of magnetic capture in comparison with conventional DNA isolation in a survey of Toxoplasma gondii in wild house mice Efficacy of magnetic capture in comparison with conventional DNA isolation in a survey of Toxoplasma gondii in wild house mice
skos:notation
RIV/62157124:16170/14:43873294!RIV15-MSM-16170___
n4:aktivita
n14:S n14:P
n4:aktivity
P(GA206/08/0640), S
n4:dodaniDat
n11:2015
n4:domaciTvurceVysledku
n5:7680244 n5:7818874
n4:druhVysledku
n13:O
n4:duvernostUdaju
n6:S
n4:entitaPredkladatele
n15:predkladatel
n4:idSjednocenehoVysledku
13820
n4:idVysledku
RIV/62157124:16170/14:43873294
n4:jazykVysledku
n16:eng
n4:klicovaSlova
quantitative PCR; magnetic capture; Mus musculus; Toxoplasma gondii
n4:klicoveSlovo
n7:Mus%20musculus n7:magnetic%20capture n7:Toxoplasma%20gondii n7:quantitative%20PCR
n4:kontrolniKodProRIV
[40B9F107CE41]
n4:obor
n9:GJ
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
5
n4:projekt
n17:GA206%2F08%2F0640
n4:rokUplatneniVysledku
n11:2014
n4:tvurceVysledku
Hofmannová, Lada Volf, Jiří Piálek, Jaroslav Baláž, Vojtěch Juránková, Jana
n12:organizacniJednotka
16170